Site-directed mutagenesis of loop L3 of sucrose porin ScrY leads to changes in substrate selectivity.

Site-directed mutagenesis of loop L3 of sucrose porin ScrY leads to changes in substrate selectivity. Research Abstract Details 

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Site-directed mutagenesis of loop L3 of sucrose porin ScrY leads to changes in substrate selectivity. Abstract Text:

C Ulmke,J Kreth,J W Lengeler,W Welte,K Schmid,

The difference in substrate selectivity of the maltodextrin (LamB) and sucrose (ScrY) porins is attributed mainly to differences in loop L3, which is supposed to constrict the lumen of the pores. We show that even a single mutation (D201Y) in loop L3 leads to a narrowing of the substrate range of ScrY to that resembling LamB. In addition, we removed the putative N-terminal coiled-coil structure of ScrY and studied the effect of this deletion on sucrose transport.

Site-directed mutagenesis of loop L3 of sucrose porin ScrY leads to changes in substrate selectivity. Publishing Authors By Initials

C Ulmke,J Kreth,JW Lengeler,W Welte,K Schmid,

For similar carbohydrates: polysaccharides: oligosaccharides: disaccharides: sucrose research abstracts see: carbohydrates: polysaccharides: oligosaccharides: disaccharides: sucrose research

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Site-directed mutagenesis of loop L3 of sucrose porin ScrY leads to changes in substrate selectivity. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of bacteriology

VOLUME: 181

Page Numbers: 1920-3

Journal Abbreviation: J. Bacteriol.

ISSN: 0021-9193

DAY: 27

MONTH: Mar

YEAR: 1999

Site-directed mutagenesis of loop L3 of sucrose porin ScrY leads to changes in substrate selectivity. Information

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LANGUAGE: eng

NlmUniqueID: 2985120

Site-directed mutagenesis of loop L3 of sucrose porin ScrY leads to changes in substrate selectivity. Keywords Mesh Terms:

KEYWORDS: Sucrose

MESH TERMS: metabolism

Chemical & Substance for Abstract: Site-directed mutagenesis of loop L3 of sucrose porin ScrY leads to changes in substrate selectivity. Information

Substance Name: Sucrose

Registry Number: 57-50-1

Grant and Affiliation Information for Site-directed mutagenesis of loop L3 of sucrose porin ScrY leads to changes in substrate selectivity.

AFFILIATION: AG Genetik, FB Biologie/Chemie, Universität Osnabrück, D-49069 Osnabrück, Germany.

Country: UNITED STATES

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MEDLINETA: J Bacteriol

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