Sinking velocities of phytoplankton measured on a stable density gradient by laser scanning.

Sinking velocities of phytoplankton measured on a stable density gradient by laser scanning. Research Abstract Details 

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Sinking velocities of phytoplankton measured on a stable density gradient by laser scanning. Abstract Text:

Anthony E Walsby,Daryl P Holland,

Two particular difficulties in measuring the sinking velocities of phytoplankton cells are preventing convection within the sedimenting medium and determining the changing depth of the cells. These problems are overcome by using a density-stabilized sedimentation column scanned by a laser. For freshwater species, a suspension of phytoplankton is layered over a vertical density gradient of Percoll solution; as the cells sink down the column their relative concentration is measured by the forward scattering of light from a laser beam that repeatedly scans up and down the column. The Percoll gradient stabilizes the column, preventing vertical mixing by convection, radiation or perturbation of density by the descending cells. Measurements were made on suspensions of 15mum polystyrene microspheres with a density of 1050kgm-3; the mean velocity was 6.28mums-1, within 1.5% of that calculated by the Stokes equation, 6.36mums-1. Measurements made on the filamentous cyanobacterium Planktothrix rubescens gave mean velocities within the theoretical range of values based on the range of size, shape, orientation and density of the particles in a modified Stokes equation. Measurements on marine phytoplankton may require density gradients prepared with other substances.

Sinking velocities of phytoplankton measured on a stable density gradient by laser scanning. Publishing Authors By Initials

AE Walsby,DP Holland,

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Sinking velocities of phytoplankton measured on a stable density gradient by laser scanning. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of the Royal Society, Interface / the Roya

VOLUME: 3

Page Numbers: 429-39

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ISSN: 1742-5689

DAY: 22

MONTH: Jun

YEAR: 2006

Sinking velocities of phytoplankton measured on a stable density gradient by laser scanning. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Sinking velocities of phytoplankton measured on a stable density gradient by laser scanning.

AFFILIATION: University of Bristol School of Biological Sciences Bristol BS8 1UG, UK.

Country: England

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MEDLINETA: J R Soc Interface

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