Single-sperm typing.

Single-sperm typing. Research Abstract Details 

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Single-sperm typing. Abstract Text:

This unit presents protocols for sperm isolation using two different methods, amplification of simple sequence-length polymorphisms (SSLP) and/or single nucleotide polymorphisms (SNP) from single cells or whole genome-amplified single cells using primer extension preamplification (PEP), and discusses the statistical analysis of sperm-typing recombination data. Newer methods for studying recombination over very short distances (a few kilobases) using total sperm DNA and allele-specific PCR are also discussed.

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PUBLICATION TYPE: Journal Article

Journal: Current protocols in human genetics / editorial bo

VOLUME: Chapter 1

Page Numbers: Unit 1.6

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ISSN: 1934-8258

DAY: 22

MONTH: May

YEAR: 2002

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LANGUAGE: eng

NlmUniqueID: 101287858

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AFFILIATION: Agricultural University of Norway, Aas, Norway.

Country: United States

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MEDLINETA: Curr Protoc Hum Genet

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