Single Mutation on the Surface of Staphylococcus aureus Sortase A Can Disrupt Its Dimerization.

Single Mutation on the Surface of Staphylococcus aureus Sortase A Can Disrupt Its Dimerization. Research Abstract Details 

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Single Mutation on the Surface of Staphylococcus aureus Sortase A Can Disrupt Its Dimerization. Abstract Text:

Jie Zhu,Changsheng Lu,Matthew Standland,Eric Lai,Gabrielle N Moreno,Aiko Umeda,Xudong Jia,Zhiwen Zhang,Jie Zhu,Changsheng Lu,Matthew Standland,Eric Lai,Gabrielle N Moreno,Aiko Umeda,Xudong Jia,Zhiwen Zhang,

Staphylococcus aureus Sortase A (SrtA) is an important Gram-positive membrane enzyme which catalyzes the anchoring of many cell surface proteins conserved with the LPXTG sequence. Recently SrtA has been demonstrated to be a dimer with a Kd of 55 muM in vitro. Herein, we show that a single point mutation of amino acid residue on the surface of SrtA can completely disrupt the dimerization. Native polyacrylamide gel electrophoresis and analytical gel filtration chromatography were used to detect the dimer-monomer equilibrium of SrtA mutants. Circular dichroism spectrum experiments were performed to study the conformational change of each SrtA mutant. An enzyme activity assay confirmed that all the SrtA mutants were active in vitro. Our results not only are important for understanding the SrtA protein self-associating mechanism but also provided the necessary starting materials for the study of sortase A pathway in vivo, which may have significant implications for discovering microbial physiology and give a potential target for novel Gram-positive antibiotics.

Single Mutation on the Surface of Staphylococcus aureus Sortase A Can Disrupt Its Dimerization. Publishing Authors By Initials

J Zhu,C Lu,M Standland,E Lai,GN Moreno,A Umeda,X Jia,Z Zhang,J Zhu,C Lu,M Standland,E Lai,GN Moreno,A Umeda,X Jia,Z Zhang,

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Single Mutation on the Surface of Staphylococcus aureus Sortase A Can Disrupt Its Dimerization. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Biochemistry

VOLUME: 47

Page Numbers: 1667-74

Journal Abbreviation: Biochemistry

ISSN: 0006-2960

DAY: 15

MONTH: 01

YEAR: 2008

Single Mutation on the Surface of Staphylococcus aureus Sortase A Can Disrupt Its Dimerization. Information

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LANGUAGE: eng

NlmUniqueID: 370623

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Grant and Affiliation Information for Single Mutation on the Surface of Staphylococcus aureus Sortase A Can Disrupt Its Dimerization.

AFFILIATION: Division of Medicinal Chemistry, College of Pharmacy, University of Texas at Austin, Austin, Texas 78712.

Country: United States

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MEDLINETA: Biochemistry

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