Single-molecule fluorescence analysis of cellular nanomachinery components.

Single-molecule fluorescence analysis of cellular nanomachinery components. Research Abstract Details 

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Single-molecule fluorescence analysis of cellular nanomachinery components. Abstract Text:

Reiner Peters,

Recent progress in proteomics suggests that the cell can be conceived as a large network of highly refined, nanomachine-like protein complexes. This working hypothesis calls for new methods capable of analyzing individual protein complexes in living cells and tissues at high speed. Here, we examine whether single-molecule fluorescence (SMF) analysis can satisfy that demand. First, recent technical progress in the visualization, localization, tracking, conformational analysis, and true resolution of individual protein complexes is highlighted. Second, results obtained by the SMF analysis of protein complexes are reviewed, focusing on the nuclear pore complex as an instructive example. We conclude that SMF methods provide powerful, indispensable tools for the structural and functional characterization of protein complexes. However, the transition from in vitro systems to living cells is in the initial stages. We discuss how current limitations in the nanoscopic analysis of living cells and tissues can be overcome to create a new paradigm, nanoscopic biomedicine.

Single-molecule fluorescence analysis of cellular nanomachinery components. Publishing Authors By Initials

R Peters,

For similar proteins research abstracts see: proteins research

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Single-molecule fluorescence analysis of cellular nanomachinery components. Journal Published:

PUBLICATION TYPE: Review

Journal: Annual review of biophysics and biomolecular struc

VOLUME: 36

Page Numbers: 371-94

Journal Abbreviation:

ISSN: 1056-8700

DAY: 3

MONTH: 12

YEAR: 2007

Single-molecule fluorescence analysis of cellular nanomachinery components. Information

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LANGUAGE: eng

NlmUniqueID: 9211097

Single-molecule fluorescence analysis of cellular nanomachinery components. Keywords Mesh Terms:

KEYWORDS: Proteins

MESH TERMS: chemistry

Chemical & Substance for Abstract: Single-molecule fluorescence analysis of cellular nanomachinery components. Information

Substance Name: Proteins

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Grant and Affiliation Information for Single-molecule fluorescence analysis of cellular nanomachinery components.

AFFILIATION: Institute of Medical Physics and Biophysics, and Center for Nanotechnology (CeNTech), University of Münster, 48149 Münster, Germany. Reiner.Peters@uni-muenster.de

Country: United States

AGENCY: United States NIGMS

GRANT: GM071329

ACRONYM: GM

MEDLINETA: Annu Rev Biophys Biomol Struct

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