Single molecule fluorescence analysis of branch migration of holliday junctions: effect of DNA sequence.

Single molecule fluorescence analysis of branch migration of holliday junctions: effect of DNA sequence. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Single molecule fluorescence analysis of branch migration of holliday junctions: effect of DNA sequence. Abstract Text:

The Holliday junction is a central intermediate in various genetic processes including homologous, site-specific recombination and DNA replication. Recent single molecule FRET experiments led to the model for branch migration as a stepwise stochastic process in which the branch migration hop is terminated by the folding of the junction. In this article, we studied the effect of the sequence on Holliday junction dynamics and branch migration process. We show that a GC pair placed at the border of the homologous region almost prevents the migration into this position. At the same time, insertion of a GC pair into the middle of the AT tract does not show this effect, however when the junction folds at this position, it resides at this position much longer time in comparison to the folding at AT pairs. Two contiguous GC pairs do not block migration as well and generally manifest the same effect as one GC pair-the junction when it folds resides at these positions for a relatively long time. The same elevated residence time was obtained for the design with the homology region that consists of only GC pairs. These data suggest a model for branch migration in which the sequence modulates the overall stochastic process of the junction dynamics and branch migration by the variability of the time that the junction dwells before making a migration hop.

Single molecule fluorescence analysis of branch migration of holliday junctions: effect of DNA sequence. Publishing Authors By Initials

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Single molecule fluorescence analysis of branch migration of holliday junctions: effect of DNA sequence. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Biophysical journal

VOLUME: 95

Page Numbers: 1239-47

Journal Abbreviation: Biophys. J.

ISSN: 1542-0086

DAY: 18

MONTH: 04

YEAR: 2008

Single molecule fluorescence analysis of branch migration of holliday junctions: effect of DNA sequence. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 370626

Single molecule fluorescence analysis of branch migration of holliday junctions: effect of DNA sequence. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Single molecule fluorescence analysis of branch migration of holliday junctions: effect of DNA sequence. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Single molecule fluorescence analysis of branch migration of holliday junctions: effect of DNA sequence.

AFFILIATION: Department of Pharmaceutical Sciences, University of Nebraska Medical Center, Nebraska Medical Center, Omaha, Nebraska 68198-6025.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Biophys J

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Single molecule fluorescence analysis of branch migration of holliday junctions: effect of DNA sequence Related Publications

• DNA structure and dynamics: an atomic force microscopy study.
• Direct visualization of the EcoRII-DNA triple synaptic complex by atomic force microscopy.
• Dynamics of synaptic SfiI-DNA complex: single-molecule fluorescence analysis.
• Effect of DNA supercoiling on the geometry of holliday junctions.
• Nanomedicine and protein misfolding diseases.
• Probing Interactions within the synaptic DNA-SfiI complex by AFM force spectroscopy.
• Single molecule fluorescence analysis of branch migration of holliday junctions: effect of DNA sequence.
• Structure, dynamics, and branch migration of a DNA Holliday junction: a single-molecule fluorescence and modeling study.