Single-molecule analysis of DNA-protein complexes using nanopores.

Single-molecule analysis of DNA-protein complexes using nanopores. Research Abstract Details 

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Single-molecule analysis of DNA-protein complexes using nanopores. Abstract Text:

Breton Hornblower,Amy Coombs,Richard D Whitaker,Anatoly Kolomeisky,Stephen J Picone,Amit Meller,Mark Akeson,

We present a method for rapid measurement of DNA-protein interactions using voltage-driven threading of single DNA molecules through a protein nanopore. Electrical force applied to individual ssDNA-exonuclease I complexes pulls the two molecules apart, while ion current probes the dissociation rate of the complex. Nanopore force spectroscopy (NFS) reveals energy barriers affecting complex dissociation. This method can be applied to other nucleic acid-protein complexes, using protein or solid-state nanopore devices.

Single-molecule analysis of DNA-protein complexes using nanopores. Publishing Authors By Initials

B Hornblower,A Coombs,RD Whitaker,A Kolomeisky,SJ Picone,A Meller,M Akeson,

For similar proteins research abstracts see: proteins research

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Single-molecule analysis of DNA-protein complexes using nanopores. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Nature methods

VOLUME: 4

Page Numbers: 315-7

Journal Abbreviation: Nat. Methods

ISSN: 1548-7091

DAY: 4

MONTH: 03

YEAR: 2007

Single-molecule analysis of DNA-protein complexes using nanopores. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101215604

Single-molecule analysis of DNA-protein complexes using nanopores. Keywords Mesh Terms:

KEYWORDS: Proteins

MESH TERMS: analysis

Chemical & Substance for Abstract: Single-molecule analysis of DNA-protein complexes using nanopores. Information

Substance Name: exodeoxyribonuclease I

Registry Number: EC 3.1.11.1

Grant and Affiliation Information for Single-molecule analysis of DNA-protein complexes using nanopores.

AFFILIATION: Department of Chemistry & Biomolecular Engineering, University of California, Santa Cruz, California 95064, USA.

Country: United States

AGENCY: United States NHGRI

GRANT: HG003703

ACRONYM: HG

MEDLINETA: Nat Methods

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