Single eye analysis and contralateral eye comparison of tear proteins in normal and dry eye model rabbits by MALDI-ToF mass spectrometry using wax-coated target plates.

Single eye analysis and contralateral eye comparison of tear proteins in normal and dry eye model rabbits by MALDI-ToF mass spectrometry using wax-coated target plates. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Single eye analysis and contralateral eye comparison of tear proteins in normal and dry eye model rabbits by MALDI-ToF mass spectrometry using wax-coated target plates. Abstract Text:

Bryan M Ham,Jean T Jacob,Richard B Cole,

A study of rabbit tear protein expression in a dry eye rabbit model was performed to determine if a pattern in expressed proteins could be identified. The uniqueness of the model allows the comparison of normal (control) eye tear protein expression with surgically induced dry eye tear protein expression in individual animals. The sensitivity of the method allows for single eye analysis. One-dimensional mini-gel electrophoresis of the tear proteins did not show substantial differences between band patterns of the normal versus the dry eye, but was used to assess the molecular weight ranges of the major proteins. Specific assignments of some of the predominant proteins were obtained by tandem mass spectrometry (MS) which showed that the lower molecular weight lipid-binding proteins (approximately 10 kDa to 36 kDa) constitute a considerable amount of the observed protein, followed in lesser quantities by the transferrins which have higher molecular weights ranging from 70 kDa to 85 kDa. Enhancement of matrix-assisted laser desorption/ionization time-of-flight (MALDI-ToF) MS linear mode analysis of intact proteins in tear fluid was demonstrated through the use of wax-coated MALDI plates and spot washing. MALDI-ToF MS analysis of the expressed tear proteins illustrates that differences between normal eye tear and dry eye tear protein content are manifested in changes in the lower molecular weight lipid-binding proteins such as lipophilin which exhibits an increase in concentration in the dry eye, and beta-2 microglobulin which undergoes a decrease.

Single eye analysis and contralateral eye comparison of tear proteins in normal and dry eye model rabbits by MALDI-ToF mass spectrometry using wax-coated target plates. Publishing Authors By Initials

BM Ham,JT Jacob,RB Cole,

For similar fluids and secretions: bodily secretions: tears research abstracts see: fluids and secretions: bodily secretions: tears research

PUBMED ID PMID:

MEDLINE DATE:

Single eye analysis and contralateral eye comparison of tear proteins in normal and dry eye model rabbits by MALDI-ToF mass spectrometry using wax-coated target plates. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Analytical and bioanalytical chemistry

VOLUME: 387

Page Numbers: 889-900

Journal Abbreviation:

ISSN: 1618-2642

DAY: 9

MONTH: 01

YEAR: 2007

Single eye analysis and contralateral eye comparison of tear proteins in normal and dry eye model rabbits by MALDI-ToF mass spectrometry using wax-coated target plates. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101134327

Single eye analysis and contralateral eye comparison of tear proteins in normal and dry eye model rabbits by MALDI-ToF mass spectrometry using wax-coated target plates. Keywords Mesh Terms:

KEYWORDS: Tears

MESH TERMS: metabolism

Chemical & Substance for Abstract: Single eye analysis and contralateral eye comparison of tear proteins in normal and dry eye model rabbits by MALDI-ToF mass spectrometry using wax-coated target plates. Information

Substance Name: tear proteins

Registry Number: 0

Grant and Affiliation Information for Single eye analysis and contralateral eye comparison of tear proteins in normal and dry eye model rabbits by MALDI-ToF mass spectrometry using wax-coated target plates.

AFFILIATION: Department of Chemistry, University of New Orleans, 2000 Lakeshore Drive, New Orleans, LA 70148, USA.

Country: Germany

AGENCY: United States NEI

GRANT: R03 EY14021

ACRONYM: EY

MEDLINETA: Anal Bioanal Chem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Single eye analysis and contralateral eye comparison of tear proteins in normal and dry eye model rabbits by MALDI-ToF mass spectrometry using wax-coated target plates Related Publications

• A multimethod examination of the stability of depressive symptoms in childhood and adolescence.
• Activation of PKR: an open and shut case?
• Agrin is required for posterior development and motor axon outgrowth and branching in embryonic zebrafish.
• Alpha-tocopheryl succinate, the most effective form of vitamin E for adjuvant cancer treatment: a review.
• Assuring quality in optometric practice.
• Bias in cross-sectional analyses of longitudinal mediation.
• Cocaine dependence and acute cocaine induce decreases of monocyte proinflammatory cytokine expression across the diurnal period: autonomic mechanisms.
• Drug abuse risk and protective factors among black urban adolescent girls: a group-randomized trial of computer-delivered mother-daughter intervention.
• Enzymatic and cellular study of a serotonin N-acetyltransferase phosphopantetheine-based prodrug.
• Estimating biomarker-based HIV incidence using prevalence data in high risk groups with missing outcomes.
• Extended function of the corpus luteum and earlier development of the second follicular wave in heifers treated with bovine somatotropin.
• Feedback-controlled stimulation enhances human paralyzed muscle performance.
• Following temperature stress, export of heat shock mRNA occurs efficiently in cells with mutations in genes normally important for mRNA export.
• Human rights and the national interest: migrants, healthcare and social justice.
• Identification of metabolites of 4,4'-methylenedianiline in vascular smooth muscle cells by liquid chromatography-electrospray tandem mass spectrometry.
• Increased cholesterol in Abeta-positive nerve terminals from Alzheimer's disease cortex.
• Inflammatory lipoproteins purified from a toxigenic and arthritogenic strain of Mycoplasma arthritidis are dependent on Toll-like receptor 2 and CD14.
• Inflammatory responses to psychological stress in fatigued breast cancer survivors: relationship to glucocorticoids.
• Mechanism of interaction of the double-stranded RNA (dsRNA) binding domain of protein kinase R with short dsRNA sequences.
• Men's issues: gender role conflict and substance abuse.
• Multivalent recombinant protein vaccine against coccidioidomycosis.
• Pharmacological studies of piperine; I. Effects of piperine on transmural nerve stimulation.
• Radiation-induced double-strand scission of the DNA of mammalian metaphase chromosomes.
• Resuscitation of the new born premature infant.
• Review: novel nonviral delivery approaches for interleukin-12 protein and gene systems: curbing toxicity and enhancing adjuvant activity.
• Single eye analysis and contralateral eye comparison of tear proteins in normal and dry eye model rabbits by MALDI-ToF mass spectrometry using wax-coated target plates.
• Temperature-humidity indices as indicators of milk production losses due to heat stress.
• The relationship between the scarf ratio and subsequent motor performance in infants born preterm.
• The retrocyclin analogue RC-101 prevents human immunodeficiency virus type 1 infection of a model human cervicovaginal tissue construct.
• Time-resolved spectroscopic studies of B12 coenzymes: comparison of the influence of solvent on the primary photolysis mechanism and geminate recombination of methyl-, ethyl-, n-propyl-, and 5'-deoxyadenosylcobalamin.