Single-drop analysis of various proteases in a cancer cell lysate using a capillary-assembled microchip.

Single-drop analysis of various proteases in a cancer cell lysate using a capillary-assembled microchip. Research Abstract Details 

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Single-drop analysis of various proteases in a cancer cell lysate using a capillary-assembled microchip. Abstract Text:

Single-drop analysis of two different real sample solutions (2 microL) while simultaneously monitoring the activity of two sets of ten different proteases on a single microfluidic device is presented. The device, called a capillary-assembled microchip (CAs-CHIP), is fabricated by embedding square glass sensing capillaries (reagent-release capillaries, RRC) in the polydimethylsiloxane (PDMS) lattice microchannel, and used for that purpose. First, the performance reliability was evaluated by measuring the fluorescence response of twenty caspase-3-sensing capillaries on a single CAs-CHIP, and a relative standard deviation of 1.5-8.2 (% RSD, n = 5 or 10) was obtained. This suggests that precise multiplexed protease-activity sensing is possible by using a single CAs-CHIP with multiple RRCs embedded. Then, using a single CAs-CHIP, real sample analysis of the activity of ten different caspases/proteases in cervical cancer (HeLa) cell lysate treated and untreated with the cell-death-inducer drug, doxorubicin, was simultaneously carried out, and a significant difference in enzyme activity between these two samples was observed. These results suggested the usefulness of the CAs-CHIP in the field of drug discovery.

Single-drop analysis of various proteases in a cancer cell lysate using a capillary-assembled microchip. Publishing Authors By Initials

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Single-drop analysis of various proteases in a cancer cell lysate using a capillary-assembled microchip. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Analytical and bioanalytical chemistry

VOLUME: 391

Page Numbers: 2507-12

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ISSN: 1618-2650

DAY: 23

MONTH: 04

YEAR: 2008

Single-drop analysis of various proteases in a cancer cell lysate using a capillary-assembled microchip. Information

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LANGUAGE: eng

NlmUniqueID: 101134327

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Grant and Affiliation Information for Single-drop analysis of various proteases in a cancer cell lysate using a capillary-assembled microchip.

AFFILIATION: Graduate School of Material Science, University of Hyogo, 3-2-1 Kouto, Kamigori-cho, Ako-gun, Hyogo, 678-1297, Japan.

Country: Germany

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MEDLINETA: Anal Bioanal Chem

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Single-drop analysis of various proteases in a cancer cell lysate using a capillary-assembled microchip Related Publications

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• Single-drop analysis of various proteases in a cancer cell lysate using a capillary-assembled microchip.