Single-bead, Single-molecule, Single-cell Fluorescence: Technologies for Drug Screening and Target Validation.

Single-bead, Single-molecule, Single-cell Fluorescence: Technologies for Drug Screening and Target Validation. Research Abstract Details 

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Single-bead, Single-molecule, Single-cell Fluorescence: Technologies for Drug Screening and Target Validation. Abstract Text:

According to many current reports, the pharmaceutical business will hit a wall over the next few years. The generic competition is expected to wipe out a double-digit billion-dollar amount from top companies' annual sales between 2007 and 2012 (Wall Street Journal, online, December 6, 2007). The industry's science engine has stalled, new blockbusters are lacking, and patent expirations are a big problem. Also, the U.S. Food and Drug Administration is pulling back on approvals, requesting larger safety studies. Among the different approaches taken throughout the industry to improve productivity and to reduce the attrition rate of compounds in the drug discovery process, an extended application of quantitative biology and biophysical methods is ranked very high. Fluorescence spectroscopy and imaging represented the main detection technologies for assays and screening methods in recent years. Today, label-free detection methods, such as isothermal titration calorimetry, differential scanning calorimetry, tandem mass spectrometry (MS(n)), light scattering, or interferometry, start to provide viable alternative readouts for physicochemical characterization of leads and hit list triaging. However, the multidimensional nature of fluorescence along with its high sensitivity and single-molecule resolution remains an unparalleled source of molecular parameters to extract all different kinds of information on molecules and ligand-protein complexes in solution. Although fluorescence-based methods are currently applied throughout the different stages of the industrial drug discovery process, they are usually applied in an unconnected way. We have developed a fully integrated hit and lead discovery process combining bead-based synthesis and screening methods with confocal fluorescence microspectroscopy. The primary on-bead screening process provides fluorescent ligands that after a multistep characterization process ultimately leads to fully mechanistically characterized cellularly validated binders and inhibitors of target protein interactions. The unlabeled small-molecular inhibitors represent chemical starting points in drug discovery and target validation.

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Single-bead, Single-molecule, Single-cell Fluorescence: Technologies for Drug Screening and Target Validation. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Annals of the New York Academy of Sciences

VOLUME: 1130

Page Numbers: 1-11

Journal Abbreviation: Ann. N. Y. Acad. Sci.

ISSN: 0077-8923

DAY: 3

MONTH: Jul

YEAR: 2008

Single-bead, Single-molecule, Single-cell Fluorescence: Technologies for Drug Screening and Target Validation. Information

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LANGUAGE: eng

NlmUniqueID: 7506858

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Grant and Affiliation Information for Single-bead, Single-molecule, Single-cell Fluorescence: Technologies for Drug Screening and Target Validation.

AFFILIATION: Novartis Institutes for BioMedical Research Vienna, Innovative Screening Technologies, Brunner Strasse 59, A-1230 Vienna, Austria. manfred.auer@novartis.com.

Country: United States

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MEDLINETA: Ann N Y Acad Sci

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