Sindbis virus entry into cells triggers apoptosis by activating sphingomyelinase, leading to the release of ceramide.

Sindbis virus entry into cells triggers apoptosis by activating sphingomyelinase, leading to the release of ceramide. Research Abstract Details 

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Sindbis virus entry into cells triggers apoptosis by activating sphingomyelinase, leading to the release of ceramide. Abstract Text:

J T Jan,S Chatterjee,D E Griffin,

Sindbis virus (SV) causes acute encephalomyelitis by infecting and inducing the death of neurons. Induction of apoptosis occurs during virus entry and involves acid-induced conformational changes in the viral surface glycoproteins and sphingomyelin (SM)-dependent fusion of the virus envelope with the endosomal membrane. We have studied neuroblastoma cells to determine how this entry process triggers cell death. Acidic sphingomyelinase was activated during entry followed by activation of neutral sphingomyelinase, SM degradation, and a sustained increase in ceramide. Ceramide-induced apoptosis and SV-induced apoptosis could be inhibited by treatment with Z-VAD-fmk, a caspase inhibitor, and by overexpression of Bcl-2, an antiapoptotic cellular protein. Acid ceramidase, expressed in a recombinant SV, decreased intracellular ceramide and protected cells from apoptosis. The data suggest that acid-induced SM-dependent virus fusion initiates the apoptotic cascade by inducing SM degradation and ceramide release.

Sindbis virus entry into cells triggers apoptosis by activating sphingomyelinase, leading to the release of ceramide. Publishing Authors By Initials

JT Jan,S Chatterjee,DE Griffin,

For similar cells: cells, cultured: tumor cells, cultured research abstracts see: cells: cells, cultured: tumor cells, cultured research

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Sindbis virus entry into cells triggers apoptosis by activating sphingomyelinase, leading to the release of ceramide. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of virology

VOLUME: 74

Page Numbers: 6425-32

Journal Abbreviation: J. Virol.

ISSN: 0022-538X

DAY: 19

MONTH: Jul

YEAR: 2000

Sindbis virus entry into cells triggers apoptosis by activating sphingomyelinase, leading to the release of ceramide. Information

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LANGUAGE: eng

NlmUniqueID: 113724

Sindbis virus entry into cells triggers apoptosis by activating sphingomyelinase, leading to the release of ceramide. Keywords Mesh Terms:

KEYWORDS: Tumor Cells, Cultured

MESH TERMS: metabolism

Chemical & Substance for Abstract: Sindbis virus entry into cells triggers apoptosis by activating sphingomyelinase, leading to the release of ceramide. Information

Substance Name: Caspases

Registry Number: EC 3.4.22.-

Grant and Affiliation Information for Sindbis virus entry into cells triggers apoptosis by activating sphingomyelinase, leading to the release of ceramide.

AFFILIATION: W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins University School of Hygiene and Public Health, Baltimore, Maryland 21205, USA.

Country: UNITED STATES

AGENCY: United States NINDS

GRANT: NS 18596

ACRONYM: NS

MEDLINETA: J Virol

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