Simultaneous microchip enzymatic measurements of blood lactate and glucose.

Simultaneous microchip enzymatic measurements of blood lactate and glucose. Research Abstract Details 

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Simultaneous microchip enzymatic measurements of blood lactate and glucose. Abstract Text:

Joseph Wang,Madhu Prakash Chatrathi,Greg E Collins,

A miniaturized capillary electrophoretic (CE) microchip device for the simultaneous measurements of lactate and glucose is described. The new microchip bioassay protocol integrates an electrophoretic separation of lactate and glucose, post-column enzymatic reactions of these metabolites with their respective oxidase enzymes, and an amperometric (anodic) detection of enzymatically-liberated hydrogen peroxide at a gold-coated thick-film carbon detector. Factors influencing the response have been examined and optimized, and the analytical performance has been characterized. Applicability of the microchip assay to clinical samples, such as serum and blood, is demonstrated. The microchip protocol obviates cross enzymatic reactions and interferences from major oxidizable constituents common to dual glucose-lactate enzyme electrodes. Such ability to rapidly separate and quantitate lactate and glucose on a small microchip platform should find important clinical and biotechnological applications.

Simultaneous microchip enzymatic measurements of blood lactate and glucose. Publishing Authors By Initials

J Wang,MP Chatrathi,GE Collins,

For similar environment and public health: environment: environment, controlled: temperature research abstracts see: environment and public health: environment: environment, controlled: temperature research

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Simultaneous microchip enzymatic measurements of blood lactate and glucose. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Analytica chimica acta

VOLUME: 585

Page Numbers: 11-6

Journal Abbreviation: Anal. Chim. Acta

ISSN: 1873-4324

DAY: 9

MONTH: 12

YEAR: 2006

Simultaneous microchip enzymatic measurements of blood lactate and glucose. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 370534

Simultaneous microchip enzymatic measurements of blood lactate and glucose. Keywords Mesh Terms:

KEYWORDS: Temperature

MESH TERMS: enzymology

Chemical & Substance for Abstract: Simultaneous microchip enzymatic measurements of blood lactate and glucose. Information

Substance Name: Hydrogen Peroxide

Registry Number: 7722-84-1

Grant and Affiliation Information for Simultaneous microchip enzymatic measurements of blood lactate and glucose.

AFFILIATION: Departments of Chemical Engineering and Chemistry and Biochemistry, Biodesign Institute, Arizona State University, Tempe, AZ 85287-5001, USA. joseph.wang@asu.edu <joseph.wang@asu.edu>

Country: Netherlands

AGENCY: United States PHS

GRANT: R01A 1056047

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MEDLINETA: Anal Chim Acta

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