Simultaneous maximization of cell permeabilization and viability in single-cell electroporation using an electrolyte-filled capillary.

Simultaneous maximization of cell permeabilization and viability in single-cell electroporation using an electrolyte-filled capillary. Research Abstract Details 

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Simultaneous maximization of cell permeabilization and viability in single-cell electroporation using an electrolyte-filled capillary. Abstract Text:

Aparna Agarwal,Imants Zudans,Owe Orwar,Stephen G Weber,

A549 cells were briefly exposed to Thioglo-1, which converts thiols to fluorescent adducts. The fluorescent cells were exposed to short (50-300 ms) electric field pulses (500 V across a 15 cm capillary) created at the tip of an electrolyte-filled capillary. Fluorescence microscopy revealed varying degrees of cell permeabilization depending on the conditions. Longer pulses and a shorter cell-capillary tip distance led to a greater decrease in the cell's fluorescence. Live/dead (calcein AM and propidium iodide) testing revealed that a certain fraction of cells died. Longer pulses and shorter cell-capillary tip distances were more deadly. An optimum condition exists at a cell-capillary tip distance of 3.5-4.5 microm and a pulse duration of 120-150 ms. At these conditions, >90% of the cells are permeabilized and 80-90% survive.

Simultaneous maximization of cell permeabilization and viability in single-cell electroporation using an electrolyte-filled capillary. Publishing Authors By Initials

A Agarwal,I Zudans,O Orwar,SG Weber,

For similar sulfhydryl compounds research abstracts see: sulfhydryl compounds research

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Simultaneous maximization of cell permeabilization and viability in single-cell electroporation using an electrolyte-filled capillary. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Analytical chemistry

VOLUME: 79

Page Numbers: 161-7

Journal Abbreviation: Anal. Chem.

ISSN: 0003-2700

DAY: 1

MONTH: Jan

YEAR: 2007

Simultaneous maximization of cell permeabilization and viability in single-cell electroporation using an electrolyte-filled capillary. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 370536

Simultaneous maximization of cell permeabilization and viability in single-cell electroporation using an electrolyte-filled capillary. Keywords Mesh Terms:

KEYWORDS: Sulfhydryl Compounds

MESH TERMS: chemistry

Chemical & Substance for Abstract: Simultaneous maximization of cell permeabilization and viability in single-cell electroporation using an electrolyte-filled capillary. Information

Substance Name: Propidium

Registry Number: 36015-30-2

Grant and Affiliation Information for Simultaneous maximization of cell permeabilization and viability in single-cell electroporation using an electrolyte-filled capillary.

AFFILIATION: Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM R01 66018

ACRONYM: GM

MEDLINETA: Anal Chem

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