Simultaneous determination of nine flavonoids and qualitative evaluation of Herba Epimedii by high performance liquid chromatography with ultraviolet detection.

Simultaneous determination of nine flavonoids and qualitative evaluation of Herba Epimedii by high performance liquid chromatography with ultraviolet detection. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Simultaneous determination of nine flavonoids and qualitative evaluation of Herba Epimedii by high performance liquid chromatography with ultraviolet detection. Abstract Text:

Hao Huang,Mingjin Liang,Xinmin Zhang,Chuan Zhang,Ziyin Shen,Weidong Zhang,Hao Huang,Mingjin Liang,Xinmin Zhang,Chuan Zhang,Ziyin Shen,Weidong Zhang,

A simple high performance liquid chromatographic method has been developed for the determination of nine flavonoids in Herba Epimedii, including quercetin-3-O-glucoside, epimedin A, hexandraside A, epimedin B, epimedin C, icariin, icariside I, icariside II, and icaritin. The chromatographic separation was performed on a Diamonsil C(18) column (5 mum, 250x4.6 mm) with gradient elution by acetonitrile-0.5% acetic buffer (adjusted to pH 5.00 with triethylamine). Methodological validation gave acceptable linearities (r(2) >0.9992) and recoveries (ranging from 98.9 to 102.4%). The limits of detection of these flavonoids ranged from 16.3 to 83.3 ng. The results indicated that the contents of flavonoids in Herba Epimedii varied significantly from habitat to habitat with contents ranging from 0.05 to 39.0 mg/g. Twenty five Herba Epimedii samples prepared from five different botanical materials were investigated by the established method, and the results showed the influence of the botanical material and the habitat on the quality of Herba Epimedii. The proposed method is simple, effective, and suitable for the evaluation of this traditional Chinese medicine.

Simultaneous determination of nine flavonoids and qualitative evaluation of Herba Epimedii by high performance liquid chromatography with ultraviolet detection. Publishing Authors By Initials

H Huang,M Liang,X Zhang,C Zhang,Z Shen,W Zhang,H Huang,M Liang,X Zhang,C Zhang,Z Shen,W Zhang,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Simultaneous determination of nine flavonoids and qualitative evaluation of Herba Epimedii by high performance liquid chromatography with ultraviolet detection. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of separation science

VOLUME: 30

Page Numbers: 3207-13

Journal Abbreviation:

ISSN: 1615-9306

DAY: 17

MONTH: Dec

YEAR: 2007

Simultaneous determination of nine flavonoids and qualitative evaluation of Herba Epimedii by high performance liquid chromatography with ultraviolet detection. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101088554

Simultaneous determination of nine flavonoids and qualitative evaluation of Herba Epimedii by high performance liquid chromatography with ultraviolet detection. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Simultaneous determination of nine flavonoids and qualitative evaluation of Herba Epimedii by high performance liquid chromatography with ultraviolet detection. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Simultaneous determination of nine flavonoids and qualitative evaluation of Herba Epimedii by high performance liquid chromatography with ultraviolet detection.

AFFILIATION: School of Pharmacy, Second Military Medical University, Shanghai, PR China. Fax: +86-21-25070386.

Country: Germany

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Sep Sci

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Simultaneous determination of nine flavonoids and qualitative evaluation of Herba Epimedii by high performance liquid chromatography with ultraviolet detection Related Publications

• A comparative study on the intercuspal occlusion among TMD patients, malocclusion patients and university students.
• Effect of retinoblastoma gene on human osteosarcoma cell line OS732.
• Enhanced antioxidant and cytoprotective abilities of vitamin E succinate is associated with a rapid uptake advantage in rat hepatocytes and mitochondria.
• Intervention by azithromycin on bleomycin-induced lung injury in rats and its mechanisms.
• Method development of adsorbed stationary phase open tubular capillary electrochromatography.
• Supercritical fluid CO2 extraction of colchicine from colchicine Autumnele liliaceae.
• Vascular endothelial growth factor gene expression in patients' ischemic skeletal muscle with diabetic foot.
• [A case of hypodermiasis with empyema]
• [Analysis of hepatitis G virus genotypes in some areas of China]
• [Autologous dendritic cells eliciting cytotoxicity of bone marrow cells against chronic granulocytic leukemia]
• [Changes of human recombination bone morphogenetic protein-2 in bone and marrow in tail suspended rats]
• [Cloning of the mouse Doc-1R gene by genomic walking]
• [Construction of recombinant adenoviruses carrying human urokinase-type plasminogen activator and its expression in hepatic stellate cells in vitro]
• [Effect of inhibition of telomerase activity on cisplatin-induced apoptosis in K562 cells]
• [Effects of electrical stimulation of lateral hypothalamic area on gastric ischemia-reperfusion injury in rats]
• [Fatal complications in traffic accident injury]
• [Indomethacin induces apoptosis of K562 cells through activation of caspases and elevation of intracellular free calcium]
• [Infection and replication of hepatitis B virus and its clearance mechanism: an overview]
• [Mechanisms underlying dopaminergic neurotoxicity in ischemic cerebral damage]
• [Physiological and pathologic implications for zero-stress state of the esophagus]
• [Protein kinases and Alzheimer's disease]
• [Retroperitoneal laparoscopic unroofing of renal cyst: use of close method]
• [Risk of leukemia among medical X-ray workers in China between 1950 and 1995]
• [Studies on association between lipoprotein lipase gene polymorphisms of Pvu II site and hypertriglyceridemics in Chinese]
• [Study on automatic segmentation of color images applied to blood cells]
• [Study on high performance liquid chromatography/indirect photometric detection of fosfomycin with acridine as detection reagent]
• [Study on the application of number fluorescence density in detecting autoantibodies titer]
• [The effects of surface morphology of calcium phosphate ceramics on apatite formation in dynamic SBF]
• [Transforming growth factor beta(1) and bone morphogenetic protein 2 induce the differentiation of odontoblasts in vitro]
• [Vector propagation algorithm based ECG simulation of bundle branch block]