Simultaneous determination of icariin, icariside II and osthole in rat plasma after oral administration of the extract of Gushudan (a Chinese compound formulation) by LC-MS/MS.

Simultaneous determination of icariin, icariside II and osthole in rat plasma after oral administration of the extract of Gushudan (a Chinese compound formulation) by LC-MS/MS. Research Abstract Details 

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Simultaneous determination of icariin, icariside II and osthole in rat plasma after oral administration of the extract of Gushudan (a Chinese compound formulation) by LC-MS/MS. Abstract Text:

Man Liu,Huiping Liu,Xiumei Lu,Chao Li,Zhili Xiong,Famei Li,Man Liu,Huiping Liu,Xiumei Lu,Chao Li,Zhili Xiong,Famei Li,

A sensitive, specific and accurate LC-MS/MS method was developed for simultaneous determination of icariin, icariside II and osthole in rat plasma. With carbamazepine as the internal standard, plasma samples were prepared by protein precipitation with acetonitrile. Analysis was carried out on an ACQUITY UPLC BEH C(18) column with a linear gradient and 0.1% aqueous acetic acid and acetonitrile were used as mobile phase. Detection was performed by means of electrospray ionization mass spectrometry in positive ion mode with multiple reaction monitoring. Linear calibration curves of icariin, icariside II and osthole were obtained over the concentration ranges of 2.00-200, 2.00-200 and 2.00-500 ng/ml, respectively. The intra- and inter-day precisions were within 8.0% and 14%, and the accuracy was from -6.0% to 9.0%. The method was successfully applied to pharmacokinetic studies of icariin, icariside II and osthole in rats after oral administration of Gushudan extract.

Simultaneous determination of icariin, icariside II and osthole in rat plasma after oral administration of the extract of Gushudan (a Chinese compound formulation) by LC-MS/MS. Publishing Authors By Initials

M Liu,H Liu,X Lu,C Li,Z Xiong,F Li,M Liu,H Liu,X Lu,C Li,Z Xiong,F Li,

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Simultaneous determination of icariin, icariside II and osthole in rat plasma after oral administration of the extract of Gushudan (a Chinese compound formulation) by LC-MS/MS. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of chromatography. B, Analytical technolog

VOLUME: 860

Page Numbers: 113-20

Journal Abbreviation: J. Chromatogr. B Analyt. Techn

ISSN: 1570-0232

DAY: 22

MONTH: 10

YEAR: 2007

Simultaneous determination of icariin, icariside II and osthole in rat plasma after oral administration of the extract of Gushudan (a Chinese compound formulation) by LC-MS/MS. Information

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LANGUAGE: eng

NlmUniqueID: 101139554

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Grant and Affiliation Information for Simultaneous determination of icariin, icariside II and osthole in rat plasma after oral administration of the extract of Gushudan (a Chinese compound formulation) by LC-MS/MS.

AFFILIATION: Department of Analytical Chemistry, Shenyang Pharmaceutical University, Shenyang 110016, PR China.

Country: Netherlands

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MEDLINETA: J Chromatogr B Analyt Technol

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