Simultaneous detection of total and allergen-specific IgE by using purified allergens in a fluorescent multiplex array.

Simultaneous detection of total and allergen-specific IgE by using purified allergens in a fluorescent multiplex array. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Simultaneous detection of total and allergen-specific IgE by using purified allergens in a fluorescent multiplex array. Abstract Text:

Eva-Maria King,Lisa D Vailes,Amy Tsay,Shama M Satinover,Martin D Chapman,Eva-Maria King,Lisa D Vailes,Amy Tsay,Shama M Satinover,Martin D Chapman,

BACKGROUND: Testing serum samples for total and allergen-specific IgE requires separate testing for each antibody and allergen specificity. OBJECTIVE: To apply fluorescent suspension array technology to allow simultaneous detection of total and allergen-specific IgE in serum in a single quantitative test. METHODS: A 7-plex suspension array for the simultaneous detection of total IgE and IgE specific to Der p 1, Der p 2, Fel d 1, Can f 1, Bet v 1, and Phl p 5 was developed, using mAb or purified allergens covalently coupled to fluorescent microspheres. The multiplex array was validated by comparing total and allergen-specific IgE levels in serum from patients with allergy with results obtained by enzyme immunoassays. RESULTS: There was a highly significant correlation between total IgE levels measured by multiplex array and fluorescent enzyme immunoassay (r = 0.97; P < .001; n = 63). Total and allergen-specific IgE levels also correlated with enzyme-linked and fluorescent enzyme immunoassay results (r = 0.44-0.94; n = 95 or 106). The multiplex array was reproducible (r = 0.86-0.99; mean coefficient of variance percentage, 12% to 25%). The sample volume required for a 7-plex assay was <20 microL per sample, compared with >400 microL in current immunoassays. CONCLUSION: The multiplex array is a high-throughput system that allows simultaneous quantification of allergen-specific and total IgE. CLINICAL IMPLICATIONS: Our results suggest that fluorescent multiplex technology will facilitate large-scale epidemiologic studies of allergic sensitization. The reduced serum volume is an advantage for pediatric studies.

Simultaneous detection of total and allergen-specific IgE by using purified allergens in a fluorescent multiplex array. Publishing Authors By Initials

EM King,LD Vailes,A Tsay,SM Satinover,MD Chapman,EM King,LD Vailes,A Tsay,SM Satinover,MD Chapman,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Simultaneous detection of total and allergen-specific IgE by using purified allergens in a fluorescent multiplex array. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: The Journal of allergy and clinical immunology

VOLUME: 120

Page Numbers: 1126-31

Journal Abbreviation: J. Allergy Clin. Immunol.

ISSN: 1097-6825

DAY: 7

MONTH: 09

YEAR: 2007

Simultaneous detection of total and allergen-specific IgE by using purified allergens in a fluorescent multiplex array. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 1275002

Simultaneous detection of total and allergen-specific IgE by using purified allergens in a fluorescent multiplex array. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Simultaneous detection of total and allergen-specific IgE by using purified allergens in a fluorescent multiplex array. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Simultaneous detection of total and allergen-specific IgE by using purified allergens in a fluorescent multiplex array.

AFFILIATION: Indoor Biotechnologies Inc, Charlottesville, VA 22903, USA. eking@inbio.com

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Allergy Clin Immunol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Simultaneous detection of total and allergen-specific IgE by using purified allergens in a fluorescent multiplex array Related Publications

• Adjunctive Immunotherapy in Combination with Lipid-associated Amphotericin B for the Treatment of Invasive Candidiasis.
• Adjuvant therapy of melanoma.
• Autosomal dominant polycystic kidney disease: time for a change?
• Bandwidth and fidelity on the NEO-Five Factor Inventory: replicability and reliability of Saucier's (1998) item cluster subcomponents.
• Coil sensitivity estimation for optimal SNR reconstruction and intensity inhomogeneity correction in phased array MR imaging.
• Come to where the flavour is: additives and pesticide residue in cigarettes.
• Concepts and preliminary data toward the realization of image-guided liver surgery.
• Context effects on the spontaneous leg movements of infants with spina bifida.
• Control of coccidiosis into the next millennium.
• Curli biogenesis and function.
• Deterministic loading of individual atoms to a high-finesse optical cavity.
• Diffraction-enhanced imaging of a porcine eye.
• Effects of norgestomet, altrenogest, and/or estradiol on follicular and hormonal characteristics of late transitional mares.
• Employee participation in workplace health promotion and wellness programs: how important are incentives, and which work best?
• Evaluating the effectiveness of four contextual features in classifying annotated clinical conditions in emergency department reports.
• Examples of mathematical modeling: tales from the crypt.
• Falling prevalence of smoking: how low can we go?
• Haptic discrimination of two-dimensional angles: influence of exploratory strategy.
• High-throughput fluorescent multiplex array for indoor allergen exposure assessment.
• In vitro polymerization of a functional Escherichia coli amyloid protein.
• Interleukin-1beta is the primary initiator of pulmonary inflammation following liver injury in mice.
• Is "YouTube" telling or selling you something? Tobacco content on the YouTube video-sharing website.
• Lipidomics reveals that adiposomes store ether lipids and mediate phospholipid traffic.
• Neighborhood Walkability and Income: Associations with Walking for Leisure and Transportation: 644: May 31 8:45 AM 9:00 AM.
• Non-surgical embryo transfer and live birth in a llama.
• Primary aldosteronism in diabetic subjects with resistant hypertension.
• Spontaneous retinal activity mediates development of ocular dominance columns and binocular receptive fields in v1.
• Target Theory Revisited: Why Physicists are Essential for Radiobiology Research.
• The 9-hexadecenoic and 11-octadecenoic acid content of natural fats and oils.
• The curli nucleator protein, CsgB, contains an amyloidogenic domain that directs CsgA polymerization.