Simplification of protein NOESY spectra using bioorganic precursor synthesis and NMR spectral editing.

Simplification of protein NOESY spectra using bioorganic precursor synthesis and NMR spectral editing. Research Abstract Details 

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Simplification of protein NOESY spectra using bioorganic precursor synthesis and NMR spectral editing. Abstract Text:

Roman Lichtenecker,Martin L Ludwiczek,Walther Schmid,Robert Konrat,

A novel method is proposed for the analysis of protein NOEs in solution. In this approach, chemically synthesized precursor compounds for the amino acids valine, leucine, and isoleucine are used for amino acid specific labeling of these hydrophobic residues. The methodology is based on a novel synthetic route to 12C,1H,2H Val, Leu, and Ile side chains selectively labeled with 13CH3 only at the terminal methyl group. In an otherwise 12C,1H labeled protein, discrimination between protons bound to 12C and 13C (or 15N) can be achieved using standard isotope-editing NMR pulse schemes. This strategy significantly relieves problems with spectral overlap through selective observation of interresidue methyl NOEs and will thus be a powerful extension of existing biomolecular NMR methodology.

Simplification of protein NOESY spectra using bioorganic precursor synthesis and NMR spectral editing. Publishing Authors By Initials

R Lichtenecker,ML Ludwiczek,W Schmid,R Konrat,

For similar proteins research abstracts see: proteins research

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Simplification of protein NOESY spectra using bioorganic precursor synthesis and NMR spectral editing. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of the American Chemical Society

VOLUME: 126

Page Numbers: 5348-9

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 5

MONTH: May

YEAR: 2004

Simplification of protein NOESY spectra using bioorganic precursor synthesis and NMR spectral editing. Information

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LANGUAGE: eng

NlmUniqueID: 7503056

Simplification of protein NOESY spectra using bioorganic precursor synthesis and NMR spectral editing. Keywords Mesh Terms:

KEYWORDS: Proteins

MESH TERMS: chemistry

Chemical & Substance for Abstract: Simplification of protein NOESY spectra using bioorganic precursor synthesis and NMR spectral editing. Information

Substance Name: Proteins

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Grant and Affiliation Information for Simplification of protein NOESY spectra using bioorganic precursor synthesis and NMR spectral editing.

AFFILIATION: Institute of Organical Chemistry, Währingerstrasse 38, A-1090 Vienna, Austria.

Country: United States

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MEDLINETA: J Am Chem Soc

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