Simple method for analysis of apolipoproteins in high density lipoproteins by high performance liquid chromatography.

Simple method for analysis of apolipoproteins in high density lipoproteins by high performance liquid chromatography. Research Abstract Details 

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Simple method for analysis of apolipoproteins in high density lipoproteins by high performance liquid chromatography. Abstract Text:

M Kinoshita,M Okazaki,H Kato,T Teramoto,T Matsushima,C Naito,H Oka,I Hara,

A simple and rapid method has been developed for the separation of apolipoproteins in high density lipoprotein (HDL) fractions by high performance liquid chromatography (HPLC) with gel permeation columns (G3000SW TSK GEL). The HPLC pattern monitored by A280 for a mixed solution of the HDL fraction (10 microliter) and an eluent buffer (200 microliter, 0.1 M sodium phosphate buffer containing 0.1% SDS) incubated at 60 degrees C for 5 min showed two completely separated peaks which corresponded to the major components of human HDL, apolipoprotein A-I and A-II. Moreover, quantitation of apolipoprotein A-I by our method was found to correlate well with that by a single radial immunodiffusion (SRID) assay.

Simple method for analysis of apolipoproteins in high density lipoproteins by high performance liquid chromatography. Publishing Authors By Initials

M Kinoshita,M Okazaki,H Kato,T Teramoto,T Matsushima,C Naito,H Oka,I Hara,

For similar lipids: lipoproteins: lipoproteins, hdl research abstracts see: lipids: lipoproteins: lipoproteins, hdl research

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Simple method for analysis of apolipoproteins in high density lipoproteins by high performance liquid chromatography. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 94

Page Numbers: 615-7

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Aug

YEAR: 1983

Simple method for analysis of apolipoproteins in high density lipoproteins by high performance liquid chromatography. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Simple method for analysis of apolipoproteins in high density lipoproteins by high performance liquid chromatography. Keywords Mesh Terms:

KEYWORDS: Lipoproteins, HDL

MESH TERMS: analysis

Chemical & Substance for Abstract: Simple method for analysis of apolipoproteins in high density lipoproteins by high performance liquid chromatography. Information

Substance Name: Lipoproteins, HDL

Registry Number: 0

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Country: JAPAN

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MEDLINETA: J Biochem

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