Simple kinetic relationships and nonspecific competition govern nuclear import rates in vivo.

Simple kinetic relationships and nonspecific competition govern nuclear import rates in vivo. Research Abstract Details 

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Simple kinetic relationships and nonspecific competition govern nuclear import rates in vivo. Abstract Text:

Benjamin L Timney,Jaclyn Tetenbaum-Novatt,Diana S Agate,Rosemary Williams,Wenzhu Zhang,Brian T Chait,Michael P Rout,

Many cargoes destined for nuclear import carry nuclear localization signals that are recognized by karyopherins (Kaps). We present methods to quantitate import rates and measure Kap and cargo concentrations in single yeast cells in vivo, providing new insights into import kinetics. By systematically manipulating the amounts, types, and affinities of Kaps and cargos, we show that import rates in vivo are simply governed by the concentrations of Kaps and their cargo and the affinity between them. These rates fit to a straightforward pump-leak model for the import process. Unexpectedly, we deduced that the main limiting factor for import is the poor ability of Kaps and cargos to find each other in the cytoplasm in a background of overwhelming nonspecific competition, rather than other more obvious candidates such as the nuclear pore complex and Ran. It is likely that most of every import round is taken up by Kaps and nuclear localization signals sampling other cytoplasmic proteins as they locate each other in the cytoplasm.

Simple kinetic relationships and nonspecific competition govern nuclear import rates in vivo. Publishing Authors By Initials

BL Timney,J Tetenbaum-Novatt,DS Agate,R Williams,W Zhang,BT Chait,MP Rout,

For similar proteins: fungal proteins: saccharomyces cerevisiae proteins research abstracts see: proteins: fungal proteins: saccharomyces cerevisiae proteins research

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Simple kinetic relationships and nonspecific competition govern nuclear import rates in vivo. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The Journal of cell biology

VOLUME: 175

Page Numbers: 579-93

Journal Abbreviation: J. Cell Biol.

ISSN: 0021-9525

DAY: 20

MONTH: Nov

YEAR: 2006

Simple kinetic relationships and nonspecific competition govern nuclear import rates in vivo. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 375356

Simple kinetic relationships and nonspecific competition govern nuclear import rates in vivo. Keywords Mesh Terms:

KEYWORDS: Saccharomyces cerevisiae Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Simple kinetic relationships and nonspecific competition govern nuclear import rates in vivo. Information

Substance Name: ribosomal protein L25

Registry Number: 0

Grant and Affiliation Information for Simple kinetic relationships and nonspecific competition govern nuclear import rates in vivo.

AFFILIATION: Laboratory of Cellular and Structural Biology and Laboratory of Gaseous Ion Chemistry, Rockefeller University, New York, NY 10021, USA.

Country: United States

AGENCY: United States NCRR

GRANT: RR00862

ACRONYM: RR

MEDLINETA: J Cell Biol

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