Silver ion induces a cyclosporine a-insensitive permeability transition in rat liver mitochondria and release of apoptogenic cytochrome C.

Silver ion induces a cyclosporine a-insensitive permeability transition in rat liver mitochondria and release of apoptogenic cytochrome C. Research Abstract Details 

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Silver ion induces a cyclosporine a-insensitive permeability transition in rat liver mitochondria and release of apoptogenic cytochrome C. Abstract Text:

Mohamad Radwan Almofti,Tomokazu Ichikawa,Kikuji Yamashita,Hiroshi Terada,Yasuo Shinohara,

Various reagents are known to open the mitochondrial permeability pore (PTP) and induce a permeability transition (PT), releasing apoptogenic proteins from the intermembrane space and triggering apoptosis. In this study, we examined the effect of Ag(+), a known cytotoxic sulfhydryl-reactive heavy metal, on isolated rat liver mitochondria. The following results were obtained: (1) Upon addition, Ag(+) instantly induced mitochondrial swelling and acceleration of respiration. (2) Cyclosporine A, a specific inhibitor of classical PT, was ineffective against the effect of Ag(+), indicating that silver ions induced non-classic PT. (3) Sulfhydryl reagents such as reduced glutathione completely inhibited the effects of Ag(+) on the mitochondria. (4) Experimental results using polyethylene glycol indicated that Ag(+) induced opening of a pore in the inner mitochondrial membrane, which could be PTP of another open state or a distinct pore. (5) Electron microscopic analysis of mitochondria treated with Ag(+) showed a novel mitochondrial configuration that was apparently different from that of normal mitochondria or Ca(2+)-treated mitochondria. (6) Ag(+) also induced the release of apoptogenic cytochrome c in a CsA-insensitive but GSH-sensitive manner. These results suggest that Ag(+) promotes a nonclassical permeability increase in the mitochondrial inner membrane that is clearly distinguishable from the classical PT and releases apoptogenic cytochrome c in a classical PT-independent manner.

Silver ion induces a cyclosporine a-insensitive permeability transition in rat liver mitochondria and release of apoptogenic cytochrome C. Publishing Authors By Initials

MR Almofti,T Ichikawa,K Yamashita,H Terada,Y Shinohara,

For similar inorganic chemicals: elements: metals, heavy: silver research abstracts see: inorganic chemicals: elements: metals, heavy: silver research

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Silver ion induces a cyclosporine a-insensitive permeability transition in rat liver mitochondria and release of apoptogenic cytochrome C. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 134

Page Numbers: 43-9

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jul

YEAR: 2003

Silver ion induces a cyclosporine a-insensitive permeability transition in rat liver mitochondria and release of apoptogenic cytochrome C. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Silver ion induces a cyclosporine a-insensitive permeability transition in rat liver mitochondria and release of apoptogenic cytochrome C. Keywords Mesh Terms:

KEYWORDS: Silver

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Silver ion induces a cyclosporine a-insensitive permeability transition in rat liver mitochondria and release of apoptogenic cytochrome C. Information

Substance Name: Cytochromes c

Registry Number: 9007-43-6

Grant and Affiliation Information for Silver ion induces a cyclosporine a-insensitive permeability transition in rat liver mitochondria and release of apoptogenic cytochrome C.

AFFILIATION: Faculty of Pharmaceutical Sciences, The University of Tokushima, Shomachi 1, Tokushima 770-8505. radwanjp@yahoo.com

Country: Japan

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MEDLINETA: J Biochem

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