Silklike materials constructed from sequences of Bombyx mori silk fibroin, fibronectin, and elastin.

Silklike materials constructed from sequences of Bombyx mori silk fibroin, fibronectin, and elastin. Research Abstract Details 

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Silklike materials constructed from sequences of Bombyx mori silk fibroin, fibronectin, and elastin. Abstract Text:

Mingying Yang,Chikako Tanaka,Kazuo Yamauchi,Kosuke Ohgo,Masato Kurokawa,Tetsuo Asakura,Mingying Yang,Chikako Tanaka,Kazuo Yamauchi,Kosuke Ohgo,Masato Kurokawa,Tetsuo Asakura,

Two silklike proteins, [TGRGDSPAGG(GAGAGS)(3)AS](5) (FS(5)) and [TGRGDSPA-(GVPGV)(2)GG(GAGAGS)(3)AS](8) (FES(8)) were designed to demonstrate the superior performance as biomaterials of silklike proteins. The former protein consists of the crystalline domain sequence, (GAGAGS)n from Bombyx mori silk fibroin and cell-adhesive sequence TGRGDSPA coming from fibronectin-containing RGD triplet. The additional sequence (GVPGV)(n) from elastin was included in the latter protein. The considerably higher cell-adhesion activities of these proteins for NHDF and VERO cells were observed by comparing with those of silklike materials without RGD sequences and also the crystalline fraction of B. mori silk fibroin. This tendency was independent of the treatments, 4.5M LiClO(4) or formic acid (FA), on silklike proteins. Their activities are also higher than those of commercial Fibronectin Ftrade mark for NHDF cell. Their structural characterization was studied using (13)C solid-state NMR. Although the overlapped peaks in usual (13)C CP/MAS NMR spectra make the detailed structural analysis difficult, the methyl resonance regions observed using dipolar dephasing NMR were very useful for the analysis. The presence of both random coil and beta-sheet structures was observed in these proteins clearly. The content of beta-sheet structure in both proteins increases after FA treatment when compared with the lyophilized samples. The production of electrospun nanofibers from their hexafluoroacetone solution was also tried. The silklike protein FES(8) could prepare nonwoven silk fibers although FS(5) could not. (c) 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008.

Silklike materials constructed from sequences of Bombyx mori silk fibroin, fibronectin, and elastin. Publishing Authors By Initials

M Yang,C Tanaka,K Yamauchi,K Ohgo,M Kurokawa,T Asakura,M Yang,C Tanaka,K Yamauchi,K Ohgo,M Kurokawa,T Asakura,

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Silklike materials constructed from sequences of Bombyx mori silk fibroin, fibronectin, and elastin. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biomedical materials research. Part A

VOLUME: 84

Page Numbers: 353-63

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ISSN: 1549-3296

DAY: 20

MONTH: Feb

YEAR: 2008

Silklike materials constructed from sequences of Bombyx mori silk fibroin, fibronectin, and elastin. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Silklike materials constructed from sequences of Bombyx mori silk fibroin, fibronectin, and elastin.

AFFILIATION: Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan.

Country: United States

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MEDLINETA: J Biomed Mater Res A

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