Silica colloidal crystals for enhanced fluorescence detection in microarrays.

Silica colloidal crystals for enhanced fluorescence detection in microarrays. Research Abstract Details 

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Silica colloidal crystals for enhanced fluorescence detection in microarrays. Abstract Text:

Suping Zheng,Hui Zhang,Eric Ross,Thai Van Le,Mary J Wirth,

Silica colloidal crystals were investigated for their potential as high surface area materials to enhance sensitivity over planar surfaces for microarrays using fluorescence detection. A relation was derived showing how crystal thickness and transmission, as well as colloid size, combine to determine the optically accessible surface area for enhancing sensitivity. Experimentally, crystals of 250-nm colloids were prepared with thicknesses determined by SEM to be 1.6, 4.2, and 11.0 microm. The material was sintered at 1000 degrees C to make it durable without affecting the crystalline structure, as confirmed by SEM. UV/visible spectrometry showed the depth of penetration (1/e) to be 8.4 microm at 488 nm for these materials. Fluorescein-labeled streptavidin and biotin were used as a model ligand-receptor pair. For the fluorescence measurements, biotin was covalently bonded to the silica surfaces, and the fluorescence was detected from the captured streptavidin-fluorescein. The observed fluorescence enhancement agreed well with the theory developed here. Compared to a planar surface, the colloidal crystal of 11.0 microm in thickness enhanced the fluorescence by nearly a factor of 80, with only a 0.3% increase in fluorescence background.

Silica colloidal crystals for enhanced fluorescence detection in microarrays. Publishing Authors By Initials

S Zheng,H Zhang,E Ross,TV Le,MJ Wirth,

For similar proteins: bacterial proteins: streptavidin research abstracts see: proteins: bacterial proteins: streptavidin research

PUBMED ID PMID:

MEDLINE DATE:

Silica colloidal crystals for enhanced fluorescence detection in microarrays. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Analytical chemistry

VOLUME: 79

Page Numbers: 3867-72

Journal Abbreviation: Anal. Chem.

ISSN: 0003-2700

DAY: 10

MONTH: 04

YEAR: 2007

Silica colloidal crystals for enhanced fluorescence detection in microarrays. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 370536

Silica colloidal crystals for enhanced fluorescence detection in microarrays. Keywords Mesh Terms:

KEYWORDS: Streptavidin

MESH TERMS: standards

Chemical & Substance for Abstract: Silica colloidal crystals for enhanced fluorescence detection in microarrays. Information

Substance Name: Streptavidin

Registry Number: 9013-20-1

Grant and Affiliation Information for Silica colloidal crystals for enhanced fluorescence detection in microarrays.

AFFILIATION: Department of Chemistry, University of Arizona, Tucson, Arizona 85721, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: R01 GM65980

ACRONYM: GM

MEDLINETA: Anal Chem

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ACCESSION NUMBER:

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