Signal sequence processing is required for the assembly of LamB trimers in the outer membrane of Escherichia coli.

Signal sequence processing is required for the assembly of LamB trimers in the outer membrane of Escherichia coli. Research Abstract Details 

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Signal sequence processing is required for the assembly of LamB trimers in the outer membrane of Escherichia coli. Abstract Text:

J H Carlson,T J Silhavy,

Proteins destined for either the periplasm or the outer membrane of Escherichia coli are translocated from the cytoplasm by a common mechanism. It is generally assumed that outer membrane proteins, such as LamB (maltoporin or lambda receptor), which are rich in beta-structure, contain additional targeting information that directs proper membrane insertion. During transit to the outer membrane, these proteins may pass, in soluble form, through the periplasm or remain membrane associated and reach their final destination via sites of inner membrane-outer membrane contact (zones of adhesion). We report lamB mutations that slow signal sequence cleavage, delay release of the protein from the inner membrane, and interfere with maltoporin biogenesis. This result is most easily explained by proposing a soluble, periplasmic LamB assembly intermediate. Additionally, we found that such lamB mutations confer several novel phenotypes consistent with an abortive attempt by the cell to target these tethered LamB molecules. These phenotypes may allow isolation of mutants in which the process of outer membrane protein targeting is altered.

Signal sequence processing is required for the assembly of LamB trimers in the outer membrane of Escherichia coli. Publishing Authors By Initials

JH Carlson,TJ Silhavy,

For similar genetic processes: recombination, genetic research abstracts see: genetic processes: recombination, genetic research

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Signal sequence processing is required for the assembly of LamB trimers in the outer membrane of Escherichia coli. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of bacteriology

VOLUME: 175

Page Numbers: 3327-34

Journal Abbreviation: J. Bacteriol.

ISSN: 0021-9193

DAY: 27

MONTH: Jun

YEAR: 1993

Signal sequence processing is required for the assembly of LamB trimers in the outer membrane of Escherichia coli. Information

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LANGUAGE: eng

NlmUniqueID: 2985120

Signal sequence processing is required for the assembly of LamB trimers in the outer membrane of Escherichia coli. Keywords Mesh Terms:

KEYWORDS: Recombination, Genetic

MESH TERMS: metabolism

Chemical & Substance for Abstract: Signal sequence processing is required for the assembly of LamB trimers in the outer membrane of Escherichia coli. Information

Substance Name: phage shock protein, Bacteria

Registry Number: 0

Grant and Affiliation Information for Signal sequence processing is required for the assembly of LamB trimers in the outer membrane of Escherichia coli.

AFFILIATION: Department of Molecular Biology, Lewis Thomas Laboratory, Princeton University, New Jersey 08544-1014.

Country: UNITED STATES

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MEDLINETA: J Bacteriol

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