Signal sequence mutations as tools for the characterization of LamB folding intermediates.

Signal sequence mutations as tools for the characterization of LamB folding intermediates. Research Abstract Details 

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Signal sequence mutations as tools for the characterization of LamB folding intermediates. Abstract Text:

Amy Rizzitello Duguay,Thomas J Silhavy,

lamBA23DA25Y and lamBA23YA25Y tether LamB to the inner membrane by blocking signal sequence processing. We isolated suppressors of lamBA23DA25Y and lamBA23YA25Y, all of which mapped within the LamB signal sequence. Most interesting were mutations that changed an amino acid with a strong positive charge to an amino acid with no charge. Further characterization of two such suppressors revealed that they produce functional LamB that is localized to the outer membrane with its entire signal sequence still attached. Biochemical analysis shows that mutant LamB monomer chases into an oligomeric species with properties different from those of wild-type LamB trimer. Because assembly of mutant LamB is slowed, these mutations provide useful tools for the characterization of LamB folding intermediates.

Signal sequence mutations as tools for the characterization of LamB folding intermediates. Publishing Authors By Initials

AR Duguay,TJ Silhavy,

For similar proteins: membrane proteins: receptors, cell surface: receptors, virus research abstracts see: proteins: membrane proteins: receptors, cell surface: receptors, virus research

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Signal sequence mutations as tools for the characterization of LamB folding intermediates. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of bacteriology

VOLUME: 184

Page Numbers: 6918-28

Journal Abbreviation: J. Bacteriol.

ISSN: 0021-9193

DAY: 27

MONTH: Dec

YEAR: 2002

Signal sequence mutations as tools for the characterization of LamB folding intermediates. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985120

Signal sequence mutations as tools for the characterization of LamB folding intermediates. Keywords Mesh Terms:

KEYWORDS: Receptors, Virus

MESH TERMS: physiology

Chemical & Substance for Abstract: Signal sequence mutations as tools for the characterization of LamB folding intermediates. Information

Substance Name: maltoporins

Registry Number: 0

Grant and Affiliation Information for Signal sequence mutations as tools for the characterization of LamB folding intermediates.

AFFILIATION: Department of Molecular Biology, Princeton University, New Jersey 08544, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM 34821

ACRONYM: GM

MEDLINETA: J Bacteriol

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