Sialoglycopeptides isolated from bovine aorta.

Sialoglycopeptides isolated from bovine aorta. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Sialoglycopeptides isolated from bovine aorta. Abstract Text:

H Saito,Z Yosizawa,

Intima-media of bovine aorta was digested with pronase, after preliminary extraction of saline (1%)-soluble substances and fat. Crude glycopeptide fraction was then obtained from the resulting complex carbohydrate fraction by fractionation with CPC (cetylpyridinium chloride). Complete separation of sialoglycopeptides was achieved by chromatography on a DEAE-cellulose column at pH 7.2 followed by repeated chromatography on a DEAE-Sephadex A-25 column at pH 5.2. Nine sialoglycopeptides (SGP 1-SGP 9) thus obtained were homogeneous on high-voltage paper electrophoresis at pH 3.5 and pH 5.2. The analytical data showed great heterogeneity of the carbohydrate chains of these preparations, although they consisted of the same monosaccharides (galactose, glucose, mannose, glucosamine, galactosamine, fucose, and sialic acid), except that SGP 1 lacked galactosamine. Heterogeneity was also observed in their peptide chains. It was noticed, however, that the contents of hexose, hexosamine, and aspartic acid of the fractions (SGP 3, SGP 4, and SGP 5) which eluted from the DEAE-Sephadex A-25 column at lower molarity of the eluting salt were higher than those of the fractions (SGP 7, SGP 8, and SGP 9) which eluted at higher molarity, while the contents of sialic acid and hydroxyamino acids were in an opposite relationship. Representative fractions (SGP 7 and SGP 9) of the latter contained many more alkali-sensitive linkages than those (SGP 3 and SGP 5) of the former, indicating the presence of many more O-glycosidic linkages between hydroxyamino acid(s) and sugar(s) in the latter than in the former. The sialoglycopeptides contained significant amounts of sialic acid, ranging from 10% (sgp 1) to 32.4% (SGP 8). The highest contents were in SGP 8 and SGP 9, which contained equimolar amounts of sialic acid and hexosamine. Furthermore, infrared spectra indicated the presence of sulfate groups in most of the sialoglycopeptides.

Sialoglycopeptides isolated from bovine aorta. Publishing Authors By Initials

H Saito,Z Yosizawa,

For similar organic chemicals: carboxylic acids: acids, acyclic: sugar acids: neuraminic acids: sialic acids research abstracts see: organic chemicals: carboxylic acids: acids, acyclic: sugar acids: neuraminic acids: sialic acids research

PUBMED ID PMID:

MEDLINE DATE:

Sialoglycopeptides isolated from bovine aorta. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 77

Page Numbers: 919-30

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: May

YEAR: 1975

Sialoglycopeptides isolated from bovine aorta. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Sialoglycopeptides isolated from bovine aorta. Keywords Mesh Terms:

KEYWORDS: Sialic Acids

MESH TERMS: isolation & purification

Chemical & Substance for Abstract: Sialoglycopeptides isolated from bovine aorta. Information

Substance Name: Sialic Acids

Registry Number: 0

Grant and Affiliation Information for Sialoglycopeptides isolated from bovine aorta.

AFFILIATION:

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Sialoglycopeptides isolated from bovine aorta Related Publications

• A glycopeptide and glycosaminoglycan-peptides isolated from rabbit gastric mucosa.
• A low sulfated keratan sulfate-like substance in rabbit femur.
• Anticoagulant activity of heparin octasaccharide.
• Biosynthesis of sulfated glycoprotein in the endometrium of rabbit uterus.
• Comparative studies on the structures of highly active and relatively inactive forms of whale heparin.
• Comparison of carbohydrate-containing substances from non-calcified and calcified portions of bovine costal cartilage.
• Enzymic degradation products of omega-heparin (whale heparin).
• Glycopeptides isolated from ovine submaxillary mucin.
• Glycosaminoglycans and glycoproteins isolated from rabbit femur.
• High performance liquid chromatography of pyridylamino derivatives of sulfated oligosaccharides in the deamination products of porcine and whale heparins.
• High performance liquid chromatography of unsaturated disaccharides produced from chondroitin sulfates by chondroitinase.
• High-performance liquid chromatography of pyridylamino derivatives of unsaturated disaccharides produced from chondroitin sulfate isomers by chondroitinases.
• Hormonal effects on the biosynthesis of sulfated glycoprotein in a microsomal fraction of the endometrium of rabbit uterus.
• Hormonal effects on the sulfation of sulfated glycoprotein in a particulate fraction of the endometrium of rabbit uterus.
• Interaction of mucopolysaccharides with glycosaminoglycans on glycosaminoglycan-bound AH-Sepharose 4B.
• Isolation and characterization of a sulfated glycoprotein from rabbit small intestine.
• Isolation and characterization of a sulfated glycoprotein from rabbit uterus.
• Isolation and characterization of human placenta fibronectin.
• Isolation and characterization of sulfated disaccharides from the deamination products of porcine heparin (alpha-heparin) and whale heparin (omega-heparin) and a comparison of the deamination products.
• Isolation and characterization of sulfated glycoproteins from the brush border fraction and the soluble fraction of rabbit small intestine.
• Metabolic rate of acidic complex saccharides in rabbit uterus under estrogenic condition.
• Proteochondroitin sulfate A in the euglobulin fraction of human plasma.
• Sialoglycopeptides isolated from bovine aorta.
• Sialoglycopeptides isolated from human arteriosclerotic tissue.
• Stability of ester sulfates in heparin to solvolysis and dilute acid treatment.
• Structures of two sulfated unsaturated disaccharides isolated from enzymatic digests of omega-heparin (whale heparin).
• Study on the factors yielding high color in the carbazole reaction with hexuronic acid-containing substances.
• Sulfated glycoproteins capable of coagulating calcium carbonate isolated from pathological human bile.
• Sulfated oligosaccharides isolated from the deamination products of heparins.
• Unsaturated disaccharides in the enzymatic digests of heparan sulfates.