Shotgun proteomic analysis of vaginal fluid from women in late pregnancy.

Shotgun proteomic analysis of vaginal fluid from women in late pregnancy. Research Abstract Details 

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Shotgun proteomic analysis of vaginal fluid from women in late pregnancy. Abstract Text:

Liquid chromatography and tandem mass spectrometry without prior fractionation (shotgun proteomics) were used to analyze vaginal fluid from patients admitted for signs and symptoms of preterm labor. The patients had an average age of 26.3 +/- 5.9 years, a gestational age of 30.5 +/- 2.5 weeks, and a median cervical dilation of 1 cm (range, 0-6 cm). None of the patients exhibited signs of vaginal infection at the time of enrollment. Shotgun proteomics yielded reproducible identifications (R = 0.973) of more than 40 proteins in vaginal fluid samples, such as plasma proteins, epithelial structural proteins, and several immunoregulatory proteins, including some that were previously linked to intra-amniotic infection. This initial characterization of the vaginal fluid proteome using a nonbiased, high-throughput technique yields reproducible results in late pregnancy. The presence of host defense proteins in vaginal fluid suggests that this technique may be useful for future study of inflammation-related preterm birth.

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Shotgun proteomic analysis of vaginal fluid from women in late pregnancy. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Reproductive sciences (Thousand Oaks, Calif.)

VOLUME: 15

Page Numbers: 263-73

Journal Abbreviation: Reprod Sci

ISSN: 1933-7205

DAY: 18

MONTH: Apr

YEAR: 2008

Shotgun proteomic analysis of vaginal fluid from women in late pregnancy. Information

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LANGUAGE: eng

NlmUniqueID: 101291249

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Grant and Affiliation Information for Shotgun proteomic analysis of vaginal fluid from women in late pregnancy.

AFFILIATION: Department of Obstetrics and Gynecology, University of Colorado Denver Health Sciences Center, Aurora, CO 80045, USA.

Country: United States

AGENCY: United States NIDDK

GRANT: P30 DK048520

ACRONYM: DK

MEDLINETA: Reprod Sci

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• Shotgun proteomic analysis of vaginal fluid from women in late pregnancy.