Short-term in vitro effects of low frequency ultrasound on odontoblast-like cells.

Short-term in vitro effects of low frequency ultrasound on odontoblast-like cells. Research Abstract Details 

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Short-term in vitro effects of low frequency ultrasound on odontoblast-like cells. Abstract Text:

Ben A Scheven,Jane L Millard,Paul R Cooper,Simon C Lea,A Damien Walmsley,Anthony J Smith,

In this study, the effects of low frequency ultrasound (US) were examined on odontoblasts, the primary cell responsible for dentinogenesis and dentine repair. An established odontoblast-like cell line, MDPC-23, was subjected to 30 kHz ultrasound at three different power settings. US induced a marginal level of cell death (3% to 4%) at lower amplitudes rising to 25% cell death at the highest power tested. The latter was reflected in a 30% decrease in cell attachment after 4 to 24 h of culture, while the number of adherent cells was reduced by approximately 10% to 15% in the lower power groups. Cell replication after 24 h, as measured by BrdU incorporation, showed no significant changes in the US-treated groups. Gene expression analyses demonstrated a moderate dose-dependent increase in the expression of GAPDH (glyseraldehyde-3-phosphate dehydrogenase)-normalised collagen type I, osteopontin (OPN), transforming growth factor-beta1 (TGFbeta1) and the heat shock protein (hsp) 70. The greatest change was found in the expression of the small hsp 25/27, which showed a two- to six-fold increase following US treatment. No significant effects were observed for alkaline phosphatase (ALP) and core-binding factor A1 (CBFA1/Runx2) expression levels. This is the first report describing US effects on odontoblasts. Further studies are warranted to elucidate US effects on odontoblast function and to evaluate US as a therapeutic application in dentine repair.

Short-term in vitro effects of low frequency ultrasound on odontoblast-like cells. Publishing Authors By Initials

BA Scheven,JL Millard,PR Cooper,SC Lea,AD Walmsley,AJ Smith,

For similar natural sciences: physics: acoustics: ultrasonics research abstracts see: natural sciences: physics: acoustics: ultrasonics research

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Short-term in vitro effects of low frequency ultrasound on odontoblast-like cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Ultrasound in medicine & biology

VOLUME: 33

Page Numbers: 1475-82

Journal Abbreviation:

ISSN: 0301-5629

DAY: 24

MONTH: 05

YEAR: 2007

Short-term in vitro effects of low frequency ultrasound on odontoblast-like cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 410553

Short-term in vitro effects of low frequency ultrasound on odontoblast-like cells. Keywords Mesh Terms:

KEYWORDS: Ultrasonics

MESH TERMS: genetics

Chemical & Substance for Abstract: Short-term in vitro effects of low frequency ultrasound on odontoblast-like cells. Information

Substance Name: Osteopontin

Registry Number: 106441-73-0

Grant and Affiliation Information for Short-term in vitro effects of low frequency ultrasound on odontoblast-like cells.

AFFILIATION: School of Dentistry, University of Birmingham, St. Chad's Queensway, Birmingham, UK. b.a.scheven@bham.ac.uk

Country: England

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MEDLINETA: Ultrasound Med Biol

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