Short peptides enhance single cell adhesion and viability on microarrays.

Short peptides enhance single cell adhesion and viability on microarrays. Research Abstract Details 

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Short peptides enhance single cell adhesion and viability on microarrays. Abstract Text:

Mandana Veiseh,Omid Veiseh,Michael C Martin,Fareid Asphahani,Miqin Zhang,

Single cell patterning holds important implications for biology, biochemistry, biotechnology, medicine, and bioinformatics. The challenge for single cell patterning is to produce small islands hosting only single cells and retaining their viability for a prolonged period of time. This study demonstrated a surface engineering approach that uses a covalently bound short peptide as a mediator to pattern cells with improved single cell adhesion and prolonged cellular viability on gold patterned SiO2 substrates. The underlying hypothesis is that cell adhesion is regulated by the type, availability, and stability of effective cell adhesion peptides, and thus covalently bound short peptides would promote cell spreading and, thus, single cell adhesion and viability. The effectiveness of this approach and the underlying mechanism for the increased probability of single cell adhesion and prolonged cell viability by short peptides were studied by comparing cellular behavior of human umbilical cord vein endothelial cells on three model surfaces whose gold electrodes were immobilized with fibronectin, physically adsorbed Arg-Glu-Asp-Val-Tyr, and covalently bound Lys-Arg-Glu-Asp-Val-Tyr, respectively. The surface chemistry and binding properties were characterized by reflectance Fourier transform infrared spectroscopy. Both short peptides were superior to fibronectin in producing adhesion of only single cells, whereas the covalently bound peptide also reduced apoptosis and necrosis of adhered cells. Controlling cell spreading by peptide binding domains to regulate apoptosis and viability represents a fundamental mechanism in cell-materials interaction and provides an effective strategy in engineering arrays of single cells.

Short peptides enhance single cell adhesion and viability on microarrays. Publishing Authors By Initials

M Veiseh,O Veiseh,MC Martin,F Asphahani,M Zhang,

For similar investigative techniques: clinical laboratory techniques: culture techniques: tissue engineering research abstracts see: investigative techniques: clinical laboratory techniques: culture techniques: tissue engineering research

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MEDLINE DATE:

Short peptides enhance single cell adhesion and viability on microarrays. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Langmuir : the ACS journal of surfaces and colloid

VOLUME: 23

Page Numbers: 4472-9

Journal Abbreviation:

ISSN: 0743-7463

DAY: 20

MONTH: 03

YEAR: 2007

Short peptides enhance single cell adhesion and viability on microarrays. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9882736

Short peptides enhance single cell adhesion and viability on microarrays. Keywords Mesh Terms:

KEYWORDS: Tissue Engineering

MESH TERMS: chemistry

Chemical & Substance for Abstract: Short peptides enhance single cell adhesion and viability on microarrays. Information

Substance Name: Silicon Dioxide

Registry Number: 7631-86-9

Grant and Affiliation Information for Short peptides enhance single cell adhesion and viability on microarrays.

AFFILIATION: Department of Materials Science & Engineering, University of Washington, Seattle, Washington 98195, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: R01 GM075095

ACRONYM: GM

MEDLINETA: Langmuir

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