Short-hairpin RNA expressed from polymerase III promoters mediates RNA interference in mosquito cells.

Short-hairpin RNA expressed from polymerase III promoters mediates RNA interference in mosquito cells. Research Abstract Details 

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Short-hairpin RNA expressed from polymerase III promoters mediates RNA interference in mosquito cells. Abstract Text:

D S Konet,J Anderson,J Piper,R Akkina,E Suchman,J Carlson,

Putative U6snRNA polymerase III (PolIII) promoters were cloned from the Anopheles gambiae and Aedes aegypti genomes. The PolIII promoters were tested for their ability to express short-hairpin RNA (shRNA) targeted to firefly luciferase and to mediate RNA interference (RNAi) knockdown of a co-transfected luciferase reporter gene vector in AG-55 Anopheles gambiae and ATC-10 Aedes aegypti cells. Promoters capable of silencing expression of the co-transfected luciferase plasmid by up to 95% in AG-55 cells and up to 75% in ATC-10 cells were identified. RNase protection experiments allowed detection of the 19 nt luciferase short-interfering RNA (siRNA) in transfected cells. These findings indicate that mosquito U6snRNA gene promoters can be used for production of shRNA to induce the RNAi response in mosquito cells.

Short-hairpin RNA expressed from polymerase III promoters mediates RNA interference in mosquito cells. Publishing Authors By Initials

DS Konet,J Anderson,J Piper,R Akkina,E Suchman,J Carlson,

For similar nucleic acids, nucleotides, and nucleosides: nucleic acids: rna: rna, nuclear: rna, small nuclear research abstracts see: nucleic acids, nucleotides, and nucleosides: nucleic acids: rna: rna, nuclear: rna, small nuclear research

PUBMED ID PMID:

MEDLINE DATE:

Short-hairpin RNA expressed from polymerase III promoters mediates RNA interference in mosquito cells. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Insect molecular biology

VOLUME: 16

Page Numbers: 199-206

Journal Abbreviation: Insect Mol. Biol.

ISSN: 0962-1075

DAY: 6

MONTH: 02

YEAR: 2007

Short-hairpin RNA expressed from polymerase III promoters mediates RNA interference in mosquito cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9303579

Short-hairpin RNA expressed from polymerase III promoters mediates RNA interference in mosquito cells. Keywords Mesh Terms:

KEYWORDS: RNA, Small Nuclear

MESH TERMS: genetics

Chemical & Substance for Abstract: Short-hairpin RNA expressed from polymerase III promoters mediates RNA interference in mosquito cells. Information

Substance Name: RNA Polymerase III

Registry Number: EC 2.7.7.-

Grant and Affiliation Information for Short-hairpin RNA expressed from polymerase III promoters mediates RNA interference in mosquito cells.

AFFILIATION: Department of Microbiology Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA.

Country: England

AGENCY: United States NIAID

GRANT: AI47139

ACRONYM: AI

MEDLINETA: Insect Mol Biol

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