Shear stress inhibition of H(2)O (2) induced p66 (Shc) phosphorylation by ASK1-JNK inactivation in endothelium.

Shear stress inhibition of H(2)O (2) induced p66 (Shc) phosphorylation by ASK1-JNK inactivation in endothelium. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Shear stress inhibition of H(2)O (2) induced p66 (Shc) phosphorylation by ASK1-JNK inactivation in endothelium. Abstract Text:

Manxiang Li,Kuan-Rau Chiou,David A Kass,

Shear stress protects endothelium from a variety of risk factors for vascular disease. Here, we demonstrate a novel mechanism whereby shear stress inhibited reactive oxygen species (ROS)-triggered signaling cascades in endothelial cells. Stimulation of bovine aortic endothelial cells (BAECs) with H(2)O(2) induced a 3.07-fold increase in p66(Shc) phosphorylation. This response was fully blocked by pretreatment of cells with specific JNK but not p38 or ERK MAP kinase inhibitor. Further study showed that knocking down of apoptosis signal-regulating kinase 1 (ASK1) by siRNA transfection in cells dramatically inhibited phosphorylation of JNK and p66(Shc) elicited by H(2)O(2). Pre-perfusion of BAECs cultured in silastic tubes with laminar flow generated by a servo-pump system for 30 min also significantly suppressed H(2)O(2)-induced phosphorylation of p66(Shc). This was accompanied by quantitatively similar inhibition of ASK1 and JNK phosphorylation and activation. These results suggested that shear stress protects endothelium against oxidant stress by suppression of ASK1-JNK-mediated p66(Shc) phosphorylation.

Shear stress inhibition of H(2)O (2) induced p66 (Shc) phosphorylation by ASK1-JNK inactivation in endothelium. Publishing Authors By Initials

M Li,KR Chiou,DA Kass,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Shear stress inhibition of H(2)O (2) induced p66 (Shc) phosphorylation by ASK1-JNK inactivation in endothelium. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Heart and vessels

VOLUME: 22

Page Numbers: 423-7

Journal Abbreviation:

ISSN: 0910-8327

DAY: 26

MONTH: 11

YEAR: 2007

Shear stress inhibition of H(2)O (2) induced p66 (Shc) phosphorylation by ASK1-JNK inactivation in endothelium. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8511258

Shear stress inhibition of H(2)O (2) induced p66 (Shc) phosphorylation by ASK1-JNK inactivation in endothelium. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Shear stress inhibition of H(2)O (2) induced p66 (Shc) phosphorylation by ASK1-JNK inactivation in endothelium. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Shear stress inhibition of H(2)O (2) induced p66 (Shc) phosphorylation by ASK1-JNK inactivation in endothelium.

AFFILIATION: Division of Cardiology, Department of Medicine, Johns Hopkins Medical Institutions, Baltimore, MD, USA, mli001@umaryland.edu.

Country: Japan

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Heart Vessels

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Shear stress inhibition of H2O 2 induced p66 Shc phosphorylation by ASK1-JNK inactivation in endothelium Related Publications

• A colorimetric microanalytical method for acetate and fluoroacetate.
• Adrenergic regulation of a key cardiac potassium channel can contribute to atrial fibrillation: evidence from an IKs transgenic mouse.
• Advanced glycation endproduct crosslink breaker (alagebrium) improves endothelial function in patients with isolated systolic hypertension.
• Altered Na+ channels promote pause-induced spontaneous diastolic activity in long QT syndrome type 3 myocytes.
• An epidemic of dyssynchrony: but what does it mean?
• Assessment of the ethical review process in Sudan.
• Comparison of direct immunofluorescence, immunoassays, and fecal flotation for detection of Cryptosporidium spp. and Giardia spp. in naturally exposed cats in 4 Northern California animal shelters.
• Comparison of initial intraocular pressure response with topical beta-adrenergic antagonists and prostaglandin analogues in African American and white individuals in the Ocular Hypertension Treatment Study.
• Comparison of ophthalmic measurements obtained via high-frequency ultrasound imaging in four species of snakes.
• Control of in vivo contraction/relaxation kinetics by myosin binding protein C: protein kinase a phosphorylation dependent and independent regulation.
• Coverage-adjusted entropy estimation.
• Determination of the in vitro susceptibility of feline tritrichomonas foetus to 5 antimicrobial agents.
• Estimation of central pressure augmentation using automated radial artery tonometry.
• Fading sodium channels in failing hearts.
• Hydrogen-deuterium exchange and selective labeling of deprotonated amino acids and peptides in the gas phase.
• Impact of arterial load and loading sequence on left ventricular tissue velocities in humans.
• Impaired chronotropic and vasodilator reserves limit exercise capacity in patients with heart failure and a preserved ejection fraction.
• In vitro methylation of predetermined regions in recombinant DNA constructs.
• Molecular basis of ranolazine block of LQT-3 mutant sodium channels: evidence for site of action.
• Mutation of an A-kinase-anchoring protein causes long-QT syndrome.
• Pathology partnerships that save lives worldwide.
• Recent integrations of mammalian Hmg retropseudogenes.
• Reversing chronic remodeling in heart failure.
• Semi-automated data processing of hydrogen exchange mass spectra using HX-Express.
• Shear stress inhibition of H(2)O (2) induced p66 (Shc) phosphorylation by ASK1-JNK inactivation in endothelium.
• Small-molecule therapies for cardiac hypertrophy: moving beneath the cell surface.
• Therapeutic potential of tetrahydrobiopterin for treating vascular and cardiac disease.
• Validated prediction model for the development of primary open-angle glaucoma in individuals with ocular hypertension.
• Wechsler's mental deterioration index in the diagnosis of organic brain disease.
• Zwitterion-dianion complexes and anion-anion clusters with negative dissociation energies.