Severely altered cholesterol homeostasis in macrophages lacking apoE and SR-BI.

Severely altered cholesterol homeostasis in macrophages lacking apoE and SR-BI. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Severely altered cholesterol homeostasis in macrophages lacking apoE and SR-BI. Abstract Text:

Patricia G Yancey,W Gray Jerome,Hong Yu,Evelyn E Griffin,Brian E Cox,Vladimir R Babaev,Sergio Fazio,MacRae F Linton,

Mice deficient in scavenger receptor class B type I (SR-BI) and apolipoprotein E (apoE) [double knockout (DKO) mice] develop dyslipidemia, accelerated atherosclerosis, and myocardial infarction, and die prematurely. We examined effects of apoE and SR-BI deficiency on macrophage cholesterol homeostasis. DKO macrophages had increased total cholesterol (TC) stores (220-380 microg/mg protein) compared with apoE-/- cells (40 microg/mg), showed significant lysosomal lipid engorgement, and increased their TC by 34% after exposure to HDL. DKO macrophages from apoE-/- mice reconstituted with DKO bone marrow showed less cholesterol accumulation (89 microg/mg), suggesting that the dyslipidemia of DKO mice explains part of the cellular cholesterol defect. However, analyses of DKO and apoE-/- macrophages from transplanted apoE-/- mice revealed a role for macrophage SR-BI, inasmuch as the TC in DKO macrophages increased by 10% in the presence of HDL, whereas apoE-/- macrophage TC decreased by 33%. After incubation with HDL, the free cholesterol (FC) increased by 29% in DKO macrophages, and decreased by 8% in apoE-/- cells, and only DKO cells had FC in large peri-nuclear pools. Similar trends were observed with apoA-I as an acceptor. Thus, the abnormal cholesterol homeostasis of DKO macrophages is due to the plasma lipid environment of DKO mice and to altered trafficking of macrophage cholesterol. Both factors are likely to contribute to the accelerated atherosclerosis in DKO mice.

Severely altered cholesterol homeostasis in macrophages lacking apoE and SR-BI. Publishing Authors By Initials

PG Yancey,WG Jerome,H Yu,EE Griffin,BE Cox,VR Babaev,S Fazio,MF Linton,

For similar diagnosis: diagnostic techniques and procedures: diagnostic imaging: microscopy: microscopy, electron research abstracts see: diagnosis: diagnostic techniques and procedures: diagnostic imaging: microscopy: microscopy, electron research

PUBMED ID PMID:

MEDLINE DATE:

Severely altered cholesterol homeostasis in macrophages lacking apoE and SR-BI. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of lipid research

VOLUME: 48

Page Numbers: 1140-9

Journal Abbreviation:

ISSN: 0022-2275

DAY: 13

MONTH: 02

YEAR: 2007

Severely altered cholesterol homeostasis in macrophages lacking apoE and SR-BI. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376606

Severely altered cholesterol homeostasis in macrophages lacking apoE and SR-BI. Keywords Mesh Terms:

KEYWORDS: Microscopy, Electron

MESH TERMS: metabolism

Chemical & Substance for Abstract: Severely altered cholesterol homeostasis in macrophages lacking apoE and SR-BI. Information

Substance Name: Cholesterol

Registry Number: 57-88-5

Grant and Affiliation Information for Severely altered cholesterol homeostasis in macrophages lacking apoE and SR-BI.

AFFILIATION: Atherosclerosis Research Unit, Division of Cardiovascular Medicine, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN, USA. patricia.g.yancey@vanderbilt.edu

Country: United States

AGENCY: United States NHLBI

GRANT: HL-65709

ACRONYM: HL

MEDLINETA: J Lipid Res

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Severely altered cholesterol homeostasis in macrophages lacking apoE and SR-BI Related Publications

• Cd36, a class B scavenger receptor, functions as a monomer to bind acetylated and oxidized low-density lipoproteins.
• Congresses, conferences, meetings, and seminars.
• Deuterium exchange as an indicator of hydrogen bond donors and acceptors.
• Eugene L. Saenger, a great man.
• Evidence for a Sav1866-like architecture for the human multidrug transporter P-glycoprotein.
• George Tievsky.
• Glenn Sheline.
• Inactivation kinetics of inoculated Escherichia coli O157:H7, Listeria monocytogenes and Salmonella enterica on strawberries by chlorine dioxide gas.
• Injuries to major arteries and their treatment.
• Its not on my watch.
• Katie at seven.
• Lots of journals.
• Macrophage expression of peroxisome proliferator-activated receptor-alpha reduces atherosclerosis in low-density lipoprotein receptor-deficient mice.
• Men in menstrual product advertising--1920-1949.
• Peripheral vascular disease.
• Psychological mechanisms in the maintenance of insomnia: arousal, distress, and sleep-related beliefs.
• Psychosocial work stressors for insomnia: a prospective study on 50-60-year-old adults in the working population.
• Radiation exposure chronicle.
• Radiology at NIH.
• Severely altered cholesterol homeostasis in macrophages lacking apoE and SR-BI.
• Surgery of the veins of the lower extremity.
• The arteriosclerotic popliteal aneurysm; a report of 14 patients treated by a preliminary lumbar sympathetic ganglionectomy and aneurysmectomy.
• The hyperfine interaction in the A (2)Pi(12) and X (2)Sigma(+) states of ytterbium monofluoride.
• The personality of peoples.
• The tree of culture.
• Throw away.
• Types of displacement in fractures of the femoral neck and observations on impaction of fractures.
• Venous thrombosis and pulmonary embolism; prophylaxis, diagnosis and treatment.
• What is radiology?
• beta-Sheet Hydrogen Bonding Patterns in Cystine Peptides.