Serum amyloid A mediates human neutrophil production of reactive oxygen species through a receptor independent of formyl peptide receptor like-1.

Serum amyloid A mediates human neutrophil production of reactive oxygen species through a receptor independent of formyl peptide receptor like-1. Research Abstract Details 

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Serum amyloid A mediates human neutrophil production of reactive oxygen species through a receptor independent of formyl peptide receptor like-1. Abstract Text:

Lena Björkman,Jennie Karlsson,Anna Karlsson,Marie-Josèphe Rabiet,Francois Boulay,Huamei Fu,Johan Bylund,Claes Dahlgren,

Serum amyloid A (SAA) is one of the acute-phase reactants, a group of plasma proteins that increases immensely in concentration during microbial infections and inflammatory conditions, and a close relationship between SAA levels and disease activity in rheumatoid arthritis (RA) has been observed. RA is an inflammatory disease, where neutrophils play important roles, and SAA is thought to participate in the inflammatory reaction by being a neutrophil chemoattractant and inducer of proinflammatory cytokines. The biological effects of SAA are reportedly mediated mainly through formyl peptide receptor like-1 (FPRL1), a G protein-coupled receptor (GPCR) belonging to the formyl peptide receptor family. Here, we confirmed the affinity of SAA for FPRL1 by showing that stably transfected HL-60 cells expressing FPRL1 were activated by SAA and that the response was inhibited by the use of the FPRL1-specific antagonist WRWWWW (WRW4). We also show that SAA activates the neutrophil NADPH-oxidase and that a reserve pool of receptors is present in storage organelles mobilized by priming agents such as TNF-alpha and LPS from Gram-negative bacteria. The induced activity was inhibited by pertussis toxin, indicating the involvement of a GPCR. However, based on FPRL1-specific desensitization and use of FPRL1 antagonist WRW4, we found the SAA-mediated effects in neutrophils to be independent of FPRL1. Based on these findings, we conclude that SAA signaling in neutrophils is mediated through a GPCR, distinct from FPRL1. Future identification and characterization of the SAA receptor could lead to development of novel, therapeutic targets for treatment of RA.

Serum amyloid A mediates human neutrophil production of reactive oxygen species through a receptor independent of formyl peptide receptor like-1. Publishing Authors By Initials

L Björkman,J Karlsson,A Karlsson,MJ Rabiet,F Boulay,H Fu,J Bylund,C Dahlgren,

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Serum amyloid A mediates human neutrophil production of reactive oxygen species through a receptor independent of formyl peptide receptor like-1. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of leukocyte biology

VOLUME: 83

Page Numbers: 245-53

Journal Abbreviation: J. Leukoc. Biol.

ISSN: 0741-5400

DAY: 5

MONTH: 11

YEAR: 2007

Serum amyloid A mediates human neutrophil production of reactive oxygen species through a receptor independent of formyl peptide receptor like-1. Information

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LANGUAGE: eng

NlmUniqueID: 8405628

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AFFILIATION: Göteborg University, Department of Rheumatology and Inflammation Research, Guldhedsgatan 10A, 41346 Göteborg, Sweden. lena.i.bjorkman@vgregion.se.

Country: United States

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MEDLINETA: J Leukoc Biol

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