Serodiagnosis of Mycobacterium avium-complex pulmonary disease using an enzyme immunoassay kit.

Serodiagnosis of Mycobacterium avium-complex pulmonary disease using an enzyme immunoassay kit. Research Abstract Details 

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Serodiagnosis of Mycobacterium avium-complex pulmonary disease using an enzyme immunoassay kit. Abstract Text:

Seigo Kitada,Kazuo Kobayashi,Satoshi Ichiyama,Shunji Takakura,Mitsunori Sakatani,Katsuhiro Suzuki,Tetsuya Takashima,Takayuki Nagai,Ikunosuke Sakurabayashi,Masami Ito,Ryoji Maekura, ,

RATIONALE: The diagnosis of Mycobacterium avium-complex pulmonary disease (MAC-PD) and/or its discrimination from pulmonary tuberculosis (TB) is sometimes complicated and time consuming. OBJECTIVES: We investigated in a six-institution multicenter study whether a serologic test based on an enzyme immunoassay (EIA) kit was useful for diagnosing MAC-PD and for distinguishing it from other lung diseases. METHODS: An EIA kit detecting serum IgA antibody to glycopeptidolipid core antigen specific for MAC was developed. Antibody levels were measured in sera from 70 patients with MAC-PD, 18 with MAC contamination, 37 with pulmonary TB, 45 with other lung diseases, and 76 healthy subjects. MEASUREMENTS AND MAIN RESULTS: Significantly higher serum IgA antibody levels were detected in patients with MAC-PD than in the other groups (P < 0.0001). Setting the cutoff point at 0.7 U/ml resulted in a sensitivity and specificity of the kit for diagnosing MAC-PD of 84.3 and 100%, respectively. Significantly higher antibody levels were also found in patients with nodular-bronchiectatic disease compared with fibrocavitary disease in MAC-PD (P < 0.05). There was a positive correlation between the extent of disease on chest computed tomography scans and the levels of antibody (r = 0.43, P < 0.05) in patients with MAC-PD. CONCLUSIONS: The EIA kit is useful for the rapid diagnosis of MAC-PD and for differentiating MAC-PD from pulmonary TB and, if validated by studies in other populations, could find wide application in clinical practice.

Serodiagnosis of Mycobacterium avium-complex pulmonary disease using an enzyme immunoassay kit. Publishing Authors By Initials

S Kitada,K Kobayashi,S Ichiyama,S Takakura,M Sakatani,K Suzuki,T Takashima,T Nagai,I Sakurabayashi,M Ito,R Maekura, ,

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Serodiagnosis of Mycobacterium avium-complex pulmonary disease using an enzyme immunoassay kit. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: American journal of respiratory and critical care

VOLUME: 177

Page Numbers: 793-7

Journal Abbreviation: Am. J. Respir. Crit. Care Med.

ISSN: 1535-4970

DAY: 13

MONTH: 12

YEAR: 2007

Serodiagnosis of Mycobacterium avium-complex pulmonary disease using an enzyme immunoassay kit. Information

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LANGUAGE: eng

NlmUniqueID: 9421642

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AFFILIATION: Department of Internal Medicine, National Hospital Organization National Toneyama Hospital, 5-1-1 Toneyama, Toyonaka-shi, Osaka 560-8552, Japan. kitadas@toneyama.hosp.go.jp

Country: United States

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MEDLINETA: Am J Respir Crit Care Med

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