Serial immunoassays in parallel on a microfluidic chip for monitoring hormone secretion from living cells.

Serial immunoassays in parallel on a microfluidic chip for monitoring hormone secretion from living cells. Research Abstract Details 

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Serial immunoassays in parallel on a microfluidic chip for monitoring hormone secretion from living cells. Abstract Text:

John F Dishinger,Robert T Kennedy,

A microfluidic chip that allows for the continuous monitoring of cellular secretions from multiple independent living samples was developed. Performance of the device was characterized through the analysis of insulin secretion from islets of Langerhans. The chip contained four individual channel networks, each capable of performing electrophoresis-based immunoassays of the perfusate from islets. In the networks, islets were housed in a chamber that was continuously perfused with pressure-driven biological media at 0.6 microL min-1. Electroosmosis was used to pull perfusate containing secreted insulin into 4-cm-long reaction channels where it mixed with fluorescein isothiocyanate-labeled insulin and anti-insulin antibody for 60 s. The reaction streams were sampled at 6.25-s intervals and analyzed in parallel using an on-chip capillary electrophoresis separation with laser-induced fluorescence detection by a scanning confocal microscope. The limit of detection for insulin was 10 nM. The device was used to complete over 1450 immunoassays of biological samples in less than 40 min, allowing the parallel monitoring of insulin release from four islets every 6.25 s.

Serial immunoassays in parallel on a microfluidic chip for monitoring hormone secretion from living cells. Publishing Authors By Initials

JF Dishinger,RT Kennedy,

For similar investigative techniques: chemistry, analytical: microchip analytical procedures: microfluidic analytical techniques research abstracts see: investigative techniques: chemistry, analytical: microchip analytical procedures: microfluidic analytical techniques research

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MEDLINE DATE:

Serial immunoassays in parallel on a microfluidic chip for monitoring hormone secretion from living cells. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Analytical chemistry

VOLUME: 79

Page Numbers: 947-54

Journal Abbreviation: Anal. Chem.

ISSN: 0003-2700

DAY: 1

MONTH: Feb

YEAR: 2007

Serial immunoassays in parallel on a microfluidic chip for monitoring hormone secretion from living cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 370536

Serial immunoassays in parallel on a microfluidic chip for monitoring hormone secretion from living cells. Keywords Mesh Terms:

KEYWORDS: Microfluidic Analytical Techniques

MESH TERMS: secretion

Chemical & Substance for Abstract: Serial immunoassays in parallel on a microfluidic chip for monitoring hormone secretion from living cells. Information

Substance Name: Insulin

Registry Number: 11061-68-0

Grant and Affiliation Information for Serial immunoassays in parallel on a microfluidic chip for monitoring hormone secretion from living cells.

AFFILIATION: Department of Chemistry and Department of Pharmacology, University of Michigan, Ann Arbor, Michigan 48109, USA.

Country: United States

AGENCY: United States NIDDK

GRANT: R37 DK046960

ACRONYM: DK

MEDLINETA: Anal Chem

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