Sequential immunization with V3 peptides from primary human immunodeficiency virus type 1 produces cross-neutralizing antibodies against primary isolates with a matching narrow-neutralization sequence motif.

Sequential immunization with V3 peptides from primary human immunodeficiency virus type 1 produces cross-neutralizing antibodies against primary isolates with a matching narrow-neutralization sequence motif. Research Abstract Details 

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Sequential immunization with V3 peptides from primary human immunodeficiency virus type 1 produces cross-neutralizing antibodies against primary isolates with a matching narrow-neutralization sequence motif. Abstract Text:

Yasuyuki Eda,Mari Takizawa,Toshio Murakami,Hiroaki Maeda,Kazuhiko Kimachi,Hiroshi Yonemura,Satoshi Koyanagi,Kouichi Shiosaki,Hirofumi Higuchi,Keiichi Makizumi,Toshihiro Nakashima,Kiyoshi Osatomi,Sachio Tokiyoshi,Shuzo Matsushita,Naoki Yamamoto,Mitsuo Honda,

An antibody response capable of neutralizing not only homologous but also heterologous forms of the CXCR4-tropic human immunodeficiency virus type 1 (HIV-1) MNp and CCR5-tropic primary isolate HIV-1 JR-CSF was achieved through sequential immunization with a combination of synthetic peptides representing HIV-1 Env V3 sequences from field and laboratory HIV-1 clade B isolates. In contrast, repeated immunization with a single V3 peptide generated antibodies that neutralized only type-specific laboratory-adapted homologous viruses. To determine whether the cross-neutralization response could be attributed to a cross-reactive antibody in the immunized animals, we isolated a monoclonal antibody, C25, which neutralized the heterologous primary viruses of HIV-1 clade B. Furthermore, we generated a humanized monoclonal antibody, KD-247, by transferring the genes of the complementary determining region of C25 into genes of the human V region of the antibody. KD-247 bound with high affinity to the "PGR" motif within the HIV-1 Env V3 tip region, and, among the established reference antibodies, it most effectively neutralized primary HIV-1 field isolates possessing the matching neutralization sequence motif, suggesting its promise for clinical applications involving passive immunizations. These results demonstrate that sequential immunization with B-cell epitope peptides may contribute to a humoral immune-based HIV vaccine strategy. Indeed, they help lay the groundwork for the development of HIV-1 vaccine strategies that use sequential immunization with biologically relevant peptides to overcome difficulties associated with otherwise poorly immunogenic epitopes.

Sequential immunization with V3 peptides from primary human immunodeficiency virus type 1 produces cross-neutralizing antibodies against primary isolates with a matching narrow-neutralization sequence motif. Publishing Authors By Initials

Y Eda,M Takizawa,T Murakami,H Maeda,K Kimachi,H Yonemura,S Koyanagi,K Shiosaki,H Higuchi,K Makizumi,T Nakashima,K Osatomi,S Tokiyoshi,S Matsushita,N Yamamoto,M Honda,

For similar peptides: peptide fragments research abstracts see: peptides: peptide fragments research

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Sequential immunization with V3 peptides from primary human immunodeficiency virus type 1 produces cross-neutralizing antibodies against primary isolates with a matching narrow-neutralization sequence motif. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of virology

VOLUME: 80

Page Numbers: 5552-62

Journal Abbreviation: J. Virol.

ISSN: 0022-538X

DAY: 3

MONTH: Jun

YEAR: 2006

Sequential immunization with V3 peptides from primary human immunodeficiency virus type 1 produces cross-neutralizing antibodies against primary isolates with a matching narrow-neutralization sequence motif. Information

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LANGUAGE: eng

NlmUniqueID: 113724

Sequential immunization with V3 peptides from primary human immunodeficiency virus type 1 produces cross-neutralizing antibodies against primary isolates with a matching narrow-neutralization sequence motif. Keywords Mesh Terms:

KEYWORDS: Peptide Fragments

MESH TERMS: immunology

Chemical & Substance for Abstract: Sequential immunization with V3 peptides from primary human immunodeficiency virus type 1 produces cross-neutralizing antibodies against primary isolates with a matching narrow-neutralization sequence motif. Information

Substance Name: Peptide Fragments

Registry Number: 0

Grant and Affiliation Information for Sequential immunization with V3 peptides from primary human immunodeficiency virus type 1 produces cross-neutralizing antibodies against primary isolates with a matching narrow-neutralization sequence motif.

AFFILIATION: AIDS Research Center, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo 162-8640, Japan.

Country: United States

AGENCY: United States NIAID

GRANT: AI 27742

ACRONYM: AI

MEDLINETA: J Virol

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