Sequential chemotactic and phagocytic activation of human polymorphonuclear neutrophils.

Sequential chemotactic and phagocytic activation of human polymorphonuclear neutrophils. Research Abstract Details 

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Sequential chemotactic and phagocytic activation of human polymorphonuclear neutrophils. Abstract Text:

Jens Martin Herrmann,John Bernardo,Heidi J Long,Kurt Seetoo,Mary E McMenamin,Eraldo L Batista,Thomas E Van Dyke,Elizabeth R Simons,

Human polymorphonuclear neutrophils (PMN) chemotax to a foreign entity. When the chemoattractants' origins are reached, specific receptors bind to the invader's surface, initiating phagocytosis, phagosome formation, and fusion with granule membranes, generating the bactericidal oxidative burst, and releasing lytic enzymes, specific peptides, and proteins. We explored the initial signaling involved in these functions by observing naïve, unprimed PMN in suspension using fluorescent indicators of cytoplasmic signals (Delta[Ca(2+)](i) and DeltapH(i)) and of bactericidal entities (oxidative species and elastase) exposed to N-formyl-methionyl-leucyl-phenylalanine (fMLP) and/or multivalent immune complexes (IC). fMLP and IC each initiate a rapid transient rise in [Ca(2+)](i), mostly from intracellular stores, simultaneously with a drop in pH(i); these are followed by a drop in [Ca(2+)](i) and a rise in pH(i), with the latter being due to a Na(+)/H(+) antiport. The impact of a second stimulation depends on the order in which stimuli are applied, on their dose, and on their nature. Provided that [Ca(2+)](i) is restored, 10(-7) M fMLP, previously shown to elicit maximal Delta[Ca(2+)](i) but no bactericidal functions, did not prevent the cells' responses with Delta[Ca(2+)](i) to a subsequent high dose of fMLP or IC; conversely, cells first exposed to 120 mug/ml IC, previously shown to elicit maximal Delta[Ca(2+)](i) and bactericidal functions, exhibited no subsequent Delta[Ca(2+)](i) or DeltapH(i) to either stimulus. While exposure to 10(-7) M fMLP, which saturates the PMN high-affinity receptor, did not elicit bactericidal release from these naïve unprimed PMN in suspension, 10(-5) M fMLP did, presumably via the low-affinity receptor, using a different Ca(2+) source.

Sequential chemotactic and phagocytic activation of human polymorphonuclear neutrophils. Publishing Authors By Initials

JM Herrmann,J Bernardo,HJ Long,K Seetoo,ME McMenamin,EL Batista,TE Van Dyke,ER Simons,

For similar proteins: membrane proteins: receptors, cell surface: receptors, immunologic: receptors, fc: receptors, igg research abstracts see: proteins: membrane proteins: receptors, cell surface: receptors, immunologic: receptors, fc: receptors, igg research

PUBMED ID PMID:

MEDLINE DATE:

Sequential chemotactic and phagocytic activation of human polymorphonuclear neutrophils. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Infection and immunity

VOLUME: 75

Page Numbers: 3989-98

Journal Abbreviation: Infect. Immun.

ISSN: 0019-9567

DAY: 25

MONTH: 05

YEAR: 2007

Sequential chemotactic and phagocytic activation of human polymorphonuclear neutrophils. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 246127

Sequential chemotactic and phagocytic activation of human polymorphonuclear neutrophils. Keywords Mesh Terms:

KEYWORDS: Receptors, IgG

MESH TERMS: immunology

Chemical & Substance for Abstract: Sequential chemotactic and phagocytic activation of human polymorphonuclear neutrophils. Information

Substance Name: Pancreatic Elastase

Registry Number: EC 3.4.21.36

Grant and Affiliation Information for Sequential chemotactic and phagocytic activation of human polymorphonuclear neutrophils.

AFFILIATION: Department of Biochemistry, Boston University School of Medicine, 80 East Concord Street, Boston, MA 02118, USA.

Country: United States

AGENCY: United States NCRR

GRANT: RR 00533

ACRONYM: RR

MEDLINETA: Infect Immun

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