[Sequence analysis and expression study of BRI gene in two lung adenocarcinoma cell lines with different metastatic potential]

[Sequence analysis and expression study of BRI gene in two lung adenocarcinoma cell lines with different metastatic potential] Research Abstract Details 

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[Sequence analysis and expression study of BRI gene in two lung adenocarcinoma cell lines with different metastatic potential] Abstract Text:

Huan-jie Yang,Yu Li,Ying-zhun Chen,Bing-jie Lu,Feng Chen,Yu Chen,Pu Li,Song-bin Fu,

OBJECTIVE: To investigate the sequence of amyloid fibrils (BRI) gene and its expression in two lung adenocarcinoma cell lines AGZY83-a and Anip973 with the same tumor origin but different metastatic potential. METHODS: DNA sequencing, sequential G banding fluorescence in situ hybridization (FISH) and Northern blot were used to analyze the sequence and expression of BRI gene was in two lung adenocarcinoma cell lines with different metastatic potential. RESULTS: The expression of BRI gene was up-regulated in the highly metastatic cell line Anip973 and was down-regulated in the low metastatic cell line AGZY83-a from which the Anip973 was derived. FISH results disclosed that in the two cell lines, the same rearrangements existed in the chromosome region where BRI gene was located, but in Anip973, amplification took place in the chromosome region where BRI gene was located. DNA sequencing results showed different mutations in the 5' untranslated region of BRI gene in the two cell lines. CONCLUSION: The above results revealed that there was no relation between BRI gene differential expression and rearrangements of chromosome. The amplification of the chromosome region where BRI gene was located and the different mutations in the 5' untranslated region of BRI gene probably contributed to the differential expression.

[Sequence analysis and expression study of BRI gene in two lung adenocarcinoma cell lines with different metastatic potential] Publishing Authors By Initials

HJ Yang,Y Li,YZ Chen,BJ Lu,F Chen,Y Chen,P Li,SB Fu,

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[Sequence analysis and expression study of BRI gene in two lung adenocarcinoma cell lines with different metastatic potential] Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Zhonghua yi xue yi chuan xue za zhi = Zhonghua yix

VOLUME: 20

Page Numbers: 292-6

Journal Abbreviation: Zhonghua Yi Xue Yi Chuan Xue Z

ISSN: 1003-9406

DAY: 6

MONTH: Aug

YEAR: 2003

[Sequence analysis and expression study of BRI gene in two lung adenocarcinoma cell lines with different metastatic potential] Information

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LANGUAGE: chi

NlmUniqueID: 9425197

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Grant and Affiliation Information for [Sequence analysis and expression study of BRI gene in two lung adenocarcinoma cell lines with different metastatic potential]

AFFILIATION: Laboratory of Medical Genetics, Harbin Medical University, Harbin, Heilongjiang, 150086 PR China.

Country: China

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MEDLINETA: Zhonghua Yi Xue Yi Chuan Xue Z

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