Separation of ribonucleotides, deoxynucleotides, cyclic nucleotides and deoxycyclic nucleotides by reversed-phase high-performance liquid chromatography.

Separation of ribonucleotides, deoxynucleotides, cyclic nucleotides and deoxycyclic nucleotides by reversed-phase high-performance liquid chromatography. Research Abstract Details 

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Separation of ribonucleotides, deoxynucleotides, cyclic nucleotides and deoxycyclic nucleotides by reversed-phase high-performance liquid chromatography. Abstract Text:

C L Willis,C K Lim,T J Peters,

A reversed-phase gradient elution system with methanol-triethylammonium phosphate buffer (83.3 mM, pH 6.0) as eluent on C(18)-bonded silica is described for the separation of 38 ribonucleotides, deoxynucleotides, cyclic nucleotides and deoxycyclic nucleotides in less than 33 min. The retention of the nucleotides can be precisely controlled by adjusting the pH, buffer concentration and methanol content in the mobile phase. The system is especially useful for the analysis of low levels of cyclic nucleotides in cells and tissues.

Separation of ribonucleotides, deoxynucleotides, cyclic nucleotides and deoxycyclic nucleotides by reversed-phase high-performance liquid chromatography. Publishing Authors By Initials

CL Willis,CK Lim,TJ Peters,

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Separation of ribonucleotides, deoxynucleotides, cyclic nucleotides and deoxycyclic nucleotides by reversed-phase high-performance liquid chromatography. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of pharmaceutical and biomedical analysis

VOLUME: 4

Page Numbers: 247-52

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ISSN: 0731-7085

DAY: 26

MONTH: 07

YEAR: 1986

Separation of ribonucleotides, deoxynucleotides, cyclic nucleotides and deoxycyclic nucleotides by reversed-phase high-performance liquid chromatography. Information

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LANGUAGE: eng

NlmUniqueID: 8309336

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Grant and Affiliation Information for Separation of ribonucleotides, deoxynucleotides, cyclic nucleotides and deoxycyclic nucleotides by reversed-phase high-performance liquid chromatography.

AFFILIATION: Division of Clinical Cell Biology, MRC Clinical Research Centre, Watford Road, Harrow, Middlesex, HA1 3UJ, UK.

Country: England

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MEDLINETA: J Pharm Biomed Anal

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