The method of separation of glucosylceramide by HPLC was reported. Glucosylceramide was perbenzoylated and separated on a packed muBondapack C18 column, using methanol as eluting solvent. The pattern obtained by HPLC closely resembled that obtained by GLC of the TMS-glucosylceramide, and reflected the molecular species of fatty acid components. This method is reproducible, and sensitive as GLC. This method also can be used for analysis of higher glycolipids.
Separation of molecular species of glucosylceramide by high performance liquid chromatography of their benzoyl derivatives. Publishing Authors By Initials
Separation of molecular species of glucosylceramide by high performance liquid chromatography of their benzoyl derivatives. Journal Published:
PUBLICATION TYPE: Journal Article
Journal: Journal of biochemistry
VOLUME: 80
Page Numbers: 1181-3
Journal Abbreviation: J. Biochem.
ISSN: 0021-924X
DAY: 19
MONTH: Nov
YEAR: 1976
Separation of molecular species of glucosylceramide by high performance liquid chromatography of their benzoyl derivatives. Information
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LANGUAGE: eng
NlmUniqueID: 376600
Separation of molecular species of glucosylceramide by high performance liquid chromatography of their benzoyl derivatives. Keywords Mesh Terms:
KEYWORDS: Spleen
MESH TERMS: isolation & purification
Chemical & Substance for Abstract: Separation of molecular species of glucosylceramide by high performance liquid chromatography of their benzoyl derivatives. Information
Substance Name: Glycosphingolipids
Registry Number: 0
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Country: JAPAN
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MEDLINETA: J Biochem
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