Separation of functionally or highly pure C2 from human plasma with Sepharose and a lectin of Euonymus europeus.

Separation of functionally or highly pure C2 from human plasma with Sepharose and a lectin of Euonymus europeus. Research Abstract Details 

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Separation of functionally or highly pure C2 from human plasma with Sepharose and a lectin of Euonymus europeus. Abstract Text:

D R Schultz,P I Arnold,

A method is described for isolating both functionally and highly pure C2 from normal human serum or plasma. Functionally pure C2 was obtained by a two-step method of ammonium sulfate (AS) fractionation of plasma or serum and chromatography on AH-Sepharose containing a bound lectin of Euonymus europeus. The functionally pure C2 can be isolated in 2 days, and is used routinely as a reagent for functional hemolytic titrations of C3 and C4 in the Clinical Immunology Laboratory. Highly pure C2 was isolated by the two-step fractionation with AS followed by chromatography on CM-cellulose, aged CNBr-activated Sepharose 4B, and AH-Sepharose-lectin. The major difficulty for isolating highly pure C2 was its separation from Factor B of the alternative complement pathway. The yield of C2 varied from 30 to 40 per cent. Following electrophoresis and staining on polyacrylamide gels, the single band was hemolytically active C2.

Separation of functionally or highly pure C2 from human plasma with Sepharose and a lectin of Euonymus europeus. Publishing Authors By Initials

DR Schultz,PI Arnold,

For similar proteins: lectins research abstracts see: proteins: lectins research

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MEDLINE DATE:

Separation of functionally or highly pure C2 from human plasma with Sepharose and a lectin of Euonymus europeus. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Acta pathologica, microbiologica, et immunologica

VOLUME: 284

Page Numbers: 59-66

Journal Abbreviation:

ISSN: 0108-0172

DAY: 12

MONTH: 02

YEAR: 1984

Separation of functionally or highly pure C2 from human plasma with Sepharose and a lectin of Euonymus europeus. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8211441

Separation of functionally or highly pure C2 from human plasma with Sepharose and a lectin of Euonymus europeus. Keywords Mesh Terms:

KEYWORDS: Lectins

MESH TERMS: isolation & purification

Chemical & Substance for Abstract: Separation of functionally or highly pure C2 from human plasma with Sepharose and a lectin of Euonymus europeus. Information

Substance Name: Complement Factor B

Registry Number: EC 3.4.21.47

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Country: DENMARK

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MEDLINETA: Acta Pathol Microbiol Immunol

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