Sensitivity enhancement in (13)C solid-state NMR of protein microcrystals by use of paramagnetic metal ions for optimizing (1)H T(1) relaxation.

Sensitivity enhancement in (13)C solid-state NMR of protein microcrystals by use of paramagnetic metal ions for optimizing (1)H T(1) relaxation. Research Abstract Details 

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Sensitivity enhancement in (13)C solid-state NMR of protein microcrystals by use of paramagnetic metal ions for optimizing (1)H T(1) relaxation. Abstract Text:

Nalinda P Wickramasinghe,Mrignayani Kotecha,Ago Samoson,Jaan Past,Yoshitaka Ishii,

We discuss a simple approach to enhance sensitivity for (13)C high-resolution solid-state NMR for proteins in microcrystals by reducing (1)H T(1) relaxation times with paramagnetic relaxation reagents. It was shown that (1)H T(1) values can be reduced from 0.4-0.8s to 60-70 ms for ubiquitin and lysozyme in D(2)O in the presence of 10 mM Cu(II)Na(2)EDTA without substantial degradation of the resolution in (13)C CPMAS spectra. Faster signal accumulation using the shorter (1)H T(1) attained by paramagnetic doping provided sensitivity enhancements of 1.4-2.9 for these proteins, reducing the experimental time for a given signal-to-noise ratio by a factor of 2.0-8.4. This approach presented here is likely to be applicable to various other proteins in order to enhance sensitivity in (13)C high-resolution solid-state NMR spectroscopy.

Sensitivity enhancement in (13)C solid-state NMR of protein microcrystals by use of paramagnetic metal ions for optimizing (1)H T(1) relaxation. Publishing Authors By Initials

NP Wickramasinghe,M Kotecha,A Samoson,J Past,Y Ishii,

For similar investigative techniques: epidemiologic methods: statistics as topic: sensitivity and specificity research abstracts see: investigative techniques: epidemiologic methods: statistics as topic: sensitivity and specificity research

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Sensitivity enhancement in (13)C solid-state NMR of protein microcrystals by use of paramagnetic metal ions for optimizing (1)H T(1) relaxation. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of magnetic resonance (San Diego, Calif. :

VOLUME: 184

Page Numbers: 350-6

Journal Abbreviation: J. Magn. Reson.

ISSN: 1090-7807

DAY: 27

MONTH: 11

YEAR: 2006

Sensitivity enhancement in (13)C solid-state NMR of protein microcrystals by use of paramagnetic metal ions for optimizing (1)H T(1) relaxation. Information

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LANGUAGE: eng

NlmUniqueID: 9707935

Sensitivity enhancement in (13)C solid-state NMR of protein microcrystals by use of paramagnetic metal ions for optimizing (1)H T(1) relaxation. Keywords Mesh Terms:

KEYWORDS: Sensitivity and Specificity

MESH TERMS: chemistry

Chemical & Substance for Abstract: Sensitivity enhancement in (13)C solid-state NMR of protein microcrystals by use of paramagnetic metal ions for optimizing (1)H T(1) relaxation. Information

Substance Name: Protons

Registry Number: 0

Grant and Affiliation Information for Sensitivity enhancement in (13)C solid-state NMR of protein microcrystals by use of paramagnetic metal ions for optimizing (1)H T(1) relaxation.

AFFILIATION: Department of Chemistry, University of Illinois at Chicago, 845 W Taylor St., Chicago, IL 60607, USA.

Country: United States

AGENCY: United States NIA

GRANT: R01 AG028490-01

ACRONYM: AG

MEDLINETA: J Magn Reson

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