Sensitivity and specificity of fluorescent immunoguided neoplasm detection in head and neck cancer xenografts.

Sensitivity and specificity of fluorescent immunoguided neoplasm detection in head and neck cancer xenografts. Research Abstract Details 

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Sensitivity and specificity of fluorescent immunoguided neoplasm detection in head and neck cancer xenografts. Abstract Text:

Brian D Kulbersh,Ryan D Duncan,J Scott Magnuson,Joni B Skipper,Kurt Zinn,Eben L Rosenthal,

OBJECTIVES: To determine whether fluorescently labeled anti-epidermal growth factor (EGFR) antibody could be used to detect residual disease and to guide surgical resections by comparing the sensitivity and specificity of optical fluorescence imaging with the sensitivity and specificity of histopathologic evaluation. DESIGN: A preclinical model of head and neck squamous cell carcinoma. SUBJECTS: Mice xenografted with SCC-1 tumor cells. INTERVENTIONS: The mice underwent systemic injection with anti-EGFR antibody (cetuximab) conjugated to an optically active fluorophore (Cy5.5). Both a subcutaneous flank model (n = 18) and an orthotopic murine model (n = 15) were used to assess for the presence of residual disease by fluorescent stereomicroscopy after subtotal resections of tumors. Histologic analysis was performed to confirm the presence or absence of disease. RESULTS: In the subcutaneous flank model, a diagnostic dose (50 microg) and therapeutic dose (250 microg) of fluorescent-labeled anti-EGFR were administered. When a diagnostic dose was given, the sensitivity was 86%, which was less than the 91% sensitivity when the higher dose was given. Tumor biopsy specimens in which disease was detected by histologic analysis but not by fluorescence (false-negative result) averaged 166 cells (range, 50-350 cells). The specificity of optical fluorescence to predict the presence of tumor in both groups was 100%. In the floor of the mouth model, we demonstrated a sensitivity of 81% and a specificity of 100%. False-negative results were obtained in a tumor fragment measuring less than 0.5 mm in diameter. CONCLUSION: These data support further investigation of fluorescently labeled anti-EGFR antibody to detect disease in the surgical setting.

Sensitivity and specificity of fluorescent immunoguided neoplasm detection in head and neck cancer xenografts. Publishing Authors By Initials

BD Kulbersh,RD Duncan,JS Magnuson,JB Skipper,K Zinn,EL Rosenthal,

For similar surgical procedures, operative: transplantation: transplantation, heterologous research abstracts see: surgical procedures, operative: transplantation: transplantation, heterologous research

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Sensitivity and specificity of fluorescent immunoguided neoplasm detection in head and neck cancer xenografts. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Archives of otolaryngology--head & neck surgery

VOLUME: 133

Page Numbers: 511-5

Journal Abbreviation: Arch. Otolaryngol. Head Neck S

ISSN: 0886-4470

DAY: 3

MONTH: May

YEAR: 2007

Sensitivity and specificity of fluorescent immunoguided neoplasm detection in head and neck cancer xenografts. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8603209

Sensitivity and specificity of fluorescent immunoguided neoplasm detection in head and neck cancer xenografts. Keywords Mesh Terms:

KEYWORDS: Transplantation, Heterologous

MESH TERMS: methods

Chemical & Substance for Abstract: Sensitivity and specificity of fluorescent immunoguided neoplasm detection in head and neck cancer xenografts. Information

Substance Name: Epidermal Growth Factor

Registry Number: 62229-50-9

Grant and Affiliation Information for Sensitivity and specificity of fluorescent immunoguided neoplasm detection in head and neck cancer xenografts.

AFFILIATION: Department of Surgery, Division of Otolaryngology-Head and Neck Surgery, University of Alabama at Birmingham, AL 35294-0012, USA.

Country: United States

AGENCY: United States NCI

GRANT: P30 CA13148

ACRONYM: CA

MEDLINETA: Arch Otolaryngol Head Neck Sur

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