Sensitive and specific method for detecting G protein-coupled receptor mRNAs.

Sensitive and specific method for detecting G protein-coupled receptor mRNAs. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Sensitive and specific method for detecting G protein-coupled receptor mRNAs. Abstract Text:

Arne Hansen,Yidong Chen,Jason M Inman,Quang N Phan,Zhi-Qing Qi,Charlie C Xiang,Miklos Palkovits,Natasha Cherman,Sergei A Kuznetsov,Pamela G Robey,Eva Mezey,Michael J Brownstein,

G protein-coupled receptors (GPCRs) mediate effects of extracellular signaling molecules in all the body's cells. These receptors are encoded by scarce mRNAs; therefore, detecting their transcripts with conventional microarrays is difficult. We present a method based on multiplex PCR and array detection of amplicons to assay GPCR gene expression with as little as 1 mug of total RNA, and using it, we profiled three human bone marrow stromal cell (BMSC) lines.

Sensitive and specific method for detecting G protein-coupled receptor mRNAs. Publishing Authors By Initials

A Hansen,Y Chen,JM Inman,QN Phan,ZQ Qi,CC Xiang,M Palkovits,N Cherman,SA Kuznetsov,PG Robey,E Mezey,MJ Brownstein,

For similar investigative techniques: epidemiologic methods: statistics as topic: sensitivity and specificity research abstracts see: investigative techniques: epidemiologic methods: statistics as topic: sensitivity and specificity research

PUBMED ID PMID:

MEDLINE DATE:

Sensitive and specific method for detecting G protein-coupled receptor mRNAs. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Nature methods

VOLUME: 4

Page Numbers: 35-7

Journal Abbreviation: Nat. Methods

ISSN: 1548-7091

DAY: 19

MONTH: 11

YEAR: 2006

Sensitive and specific method for detecting G protein-coupled receptor mRNAs. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101215604

Sensitive and specific method for detecting G protein-coupled receptor mRNAs. Keywords Mesh Terms:

KEYWORDS: Sensitivity and Specificity

MESH TERMS: genetics

Chemical & Substance for Abstract: Sensitive and specific method for detecting G protein-coupled receptor mRNAs. Information

Substance Name: Receptors, G-Protein-Coupled

Registry Number: 0

Grant and Affiliation Information for Sensitive and specific method for detecting G protein-coupled receptor mRNAs.

AFFILIATION: Craniofacial and Skeletal Diseases Branch, National Institute of Dental and Craniofacial Research, National Institute of Health, Department of Health and Human Services, Bethesda, Maryland 20892, USA. arnehansen@nidcr.nih.gov

Country: United States

AGENCY: United States PHS

GRANT: N01-C0-12400

ACRONYM:

MEDLINETA: Nat Methods

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Sensitive and specific method for detecting G protein-coupled receptor mRNAs Related Publications

• "Rudimentary polydactyly": an amputation neuroma.
• A new insight into the cellular regulation of aqueous outflow: how trabecular meshwork endothelial cells drive a mechanism that regulates the permeability of Schlemm's canal endothelial cells.
• AIDS and the executive directo. Perspectives in crisis management.
• Addressing heart disease and stroke prevention through comprehensive population-level approaches.
• Age-related trends in the timeliness and prediction of medical visits, hospitalizations and deaths due to pneumonia and influenza, British Columbia, Canada, 1998-2004.
• Analysis of Isolated YAC Clones.
• Bilateral odontogenous sinuses.
• Delayed hypersensitivity skin reactions in the elderly.
• Dermatohistopathology of chronic gonococcal sepsis.
• Desmoplastic malignant melanoma.
• Differentiation of cultured pre-adipose cells: a probability model.
• Estimating excess mortality in post-invasion Iraq.
• Fixed eruptions due to an ephedrine isomer.
• Geriatric education in a teaching hospital: the role of geriatric services.
• Letter: Idiopathic pigmentation of the upper back.
• Multiple agminated juvenile melanoma.
• Overview of Strategies for Screening YAC Libraries and Analyzing YAC Clones.
• Pilar sheath acanthoma.
• Prevalence of anemia and correlation of hemoglobin with age in a geriatric screening clinic population.
• Proceedings: Eccrine hidrocystoma.
• Psoriasis and diabetes mellitus.
• Re: "implant infection in porous orbital implants".
• Replacing revolving door: a collaborative approach to treating individuals in crisis.
• Sensitive and specific method for detecting G protein-coupled receptor mRNAs.
• Steatocystoma simplex. A solitary steatocystoma.
• The new International Health Regulations: considerations for global public health surveillance.
• Tyson's "glands." Ectopic sebaceous glands and papillomatosis penis.