Self-assembly of recombinant amphiphilic oligopeptides into vesicles.

Self-assembly of recombinant amphiphilic oligopeptides into vesicles. Research Abstract Details 

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Self-assembly of recombinant amphiphilic oligopeptides into vesicles. Abstract Text:

Albert J van Hell,Cristina I C A Costa,Frits M Flesch,Marc Sutter,Wim Jiskoot,Daan J A Crommelin,Wim E Hennink,Enrico Mastrobattista,

The aim of the present study was to design amphiphilic oligopeptides that can self-assemble into vesicular structures. The ratio of hydrophilic to hydrophobic block length was varied, and peptides were designed to have a hydrophobic tail in which the bulkiness of the amino acid side groups increases toward the hydrophilic domain (Ac-Ala-Ala-Val-Val-Leu-Leu-Leu-Trp-Glu(2/7)-COOH). These peptides were recombinantly produced in bacteria as an alternative to solid-phase synthesis. We demonstrate with different complementary techniques (dynamic and static light scattering, tryptophan fluorescence anisotropy, and electron microscopy) that these amphiphilic peptides spontaneously form vesicles with a radius of approximately 60 nm and a low polydispersity when dispersed in aqueous solution at neutral pH. Morphology and size of the vesicles were relatively insensitive to the variations in hydrophilic block length. Exposure to acidic pH resulted in formation of visible aggregates, which could be fully reversed to vesicles upon pH neutralization. In addition, it was demonstrated that water-soluble molecules can be entrapped inside these peptide vesicles. Such peptide vesicles may find applications as biodegradable drug delivery systems with a pH-dependent release profile.

Self-assembly of recombinant amphiphilic oligopeptides into vesicles. Publishing Authors By Initials

AJ van Hell,CI Costa,FM Flesch,M Sutter,W Jiskoot,DJ Crommelin,WE Hennink,E Mastrobattista,

For similar chemical actions and uses: specialty uses of chemicals: surface-active agents research abstracts see: chemical actions and uses: specialty uses of chemicals: surface-active agents research

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Self-assembly of recombinant amphiphilic oligopeptides into vesicles. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Biomacromolecules

VOLUME: 8

Page Numbers: 2753-61

Journal Abbreviation: Biomacromolecules

ISSN: 1525-7797

DAY: 14

MONTH: 08

YEAR: 2007

Self-assembly of recombinant amphiphilic oligopeptides into vesicles. Information

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LANGUAGE: eng

NlmUniqueID: 100892849

Self-assembly of recombinant amphiphilic oligopeptides into vesicles. Keywords Mesh Terms:

KEYWORDS: Surface-Active Agents

MESH TERMS: chemistry

Chemical & Substance for Abstract: Self-assembly of recombinant amphiphilic oligopeptides into vesicles. Information

Substance Name: fluorexon

Registry Number: 1461-15-0

Grant and Affiliation Information for Self-assembly of recombinant amphiphilic oligopeptides into vesicles.

AFFILIATION: Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, P.O. Box 80082, 3508 TB Utrecht, The Netherlands.

Country: United States

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MEDLINETA: Biomacromolecules

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