Selective tropism of liver stem cells to hepatocellular carcinoma in vivo.

Selective tropism of liver stem cells to hepatocellular carcinoma in vivo. Research Abstract Details 

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Selective tropism of liver stem cells to hepatocellular carcinoma in vivo. Abstract Text:

Xiao-Gang Zhong,Sheng He,Wu Yin,Jing-Yu Deng,Bo Cheng,

AIM: To investigate the selective tropism of liver stem cells to hepatocellular carcinoma (HCC) in an animal model and its feasibility as a vector to deliver therapeutic genes for targeted therapy of HCC. METHODS: WB-F344, a kind of rat liver stem cell, was infected with recombinant virus to establish a cell line with stable, high-level expressing enhanced green fluorescent protein (EGFP). An animal model of HCC in Wistar rats was established by implanting HCC cells (CBRH7919) combined with an immunosuppressive drug. EGFP labeled liver stem cells were injected into caudal veins of the animals and distribution was observed at different time points after injection. SDF-1 and c-kit expression in non-tumor liver and tumor tissue were analysed by immunohistochemistry for the relationshiop between the expression and migration of liver stem cells. Furthermore, hepatic stem cells were injected via the portal vein, hepatic artery, caudal vein, or directly into the pericancerous liver tissue, respectively, and effects on migration, localization, and proliferation of the hepatic stem cells within the tumor tissue were observed and analyzed. RESULTS: Recombinant adenovirus could deliver the EGFP gene to hepatic stem cells. A new stem cell line, named WB-EGFP, was established that stably expressed EGFP. WB-EGFP cells still showed selective tropism towards HCC and EGFP expression was stable in vivo. According to immunohistochemistry results, SDF-1 may not be related to the mechanisms of tropism of hepatic stem cells. Different application sites affected the distribution of liver stem cells. Injection via the portal vein was superior with regard to selective migration, localization, and proliferation of the hepatic stem cells within the tumor tissue. CONCLUSION: Liver stem cells have the biological behavior of selective migration to HCC in vivo and they could localize and proliferate within HCC tissue stably expressing the target gene. Liver stem cells are a potential tool for a targeted gene therapy of HCC.

Selective tropism of liver stem cells to hepatocellular carcinoma in vivo. Publishing Authors By Initials

XG Zhong,S He,W Yin,JY Deng,B Cheng,

For similar biological phenomena, cell phenomena, and immunity: plant physiology: tropism research abstracts see: biological phenomena, cell phenomena, and immunity: plant physiology: tropism research

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Selective tropism of liver stem cells to hepatocellular carcinoma in vivo. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: World journal of gastroenterology : WJG

VOLUME: 13

Page Numbers: 3886-91

Journal Abbreviation: World J. Gastroenterol.

ISSN: 1007-9327

DAY: 28

MONTH: Jul

YEAR: 2007

Selective tropism of liver stem cells to hepatocellular carcinoma in vivo. Information

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LANGUAGE: eng

NlmUniqueID: 100883448

Selective tropism of liver stem cells to hepatocellular carcinoma in vivo. Keywords Mesh Terms:

KEYWORDS: Tropism

MESH TERMS: physiology

Chemical & Substance for Abstract: Selective tropism of liver stem cells to hepatocellular carcinoma in vivo. Information

Substance Name: Green Fluorescent Proteins

Registry Number: 147336-22-9

Grant and Affiliation Information for Selective tropism of liver stem cells to hepatocellular carcinoma in vivo.

AFFILIATION: Department of General Surgery, The People's Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, Guangxi Zhuang Autonomous Region, China. zhong.xiaogang@163.com

Country: China

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MEDLINETA: World J Gastroenterol

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