Selective sparing of goblet cells and paneth cells in the intestine of methotrexate-treated rats.

Selective sparing of goblet cells and paneth cells in the intestine of methotrexate-treated rats. Research Abstract Details 

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Selective sparing of goblet cells and paneth cells in the intestine of methotrexate-treated rats. Abstract Text:

M Verburg,I B Renes,H P Meijer,J A Taminiau,H A ,A W Einerhand,J Dekker,

Proliferation, differentiation, and cell death were studied in small intestinal and colonic epithelia of rats after treatment with methotrexate. Days 1-2 after treatment were characterized by decreased proliferation, increased apoptosis, and decreased numbers and depths of small intestinal crypts in a proximal-to-distal decreasing gradient along the small intestine. The remaining crypt epithelium appeared flattened, except for Paneth cells, in which lysozyme protein and mRNA expression was increased. Regeneration through increased proliferation during days 3-4 coincided with villus atrophy, showing decreased numbers of villus enterocytes and decreased expression of the enterocyte-specific genes sucrase-isomaltase and carbamoyl phosphate synthase I. Remarkably, goblet cells were spared at villus tips and remained functional, displaying Muc2 and trefoil factor 3 expression. On days 8-10, all parameters had returned to normal in the whole small intestine. No methotrexate-induced changes were seen in epithelial morphology, proliferation, apoptosis, Muc2, and TFF3 immunostaining in the colon. The observed small intestinal sparing of Paneth cells and goblet cells following exposure to methotrexate is likely to contribute to epithelial defense during increased vulnerability of the intestinal epithelium.

Selective sparing of goblet cells and paneth cells in the intestine of methotrexate-treated rats. Publishing Authors By Initials

M Verburg,IB Renes,HP Meijer,JA Taminiau,HA ,AW Einerhand,J Dekker,

For similar biological phenomena, cell phenomena, and immunity: biological phenomena: regeneration research abstracts see: biological phenomena, cell phenomena, and immunity: biological phenomena: regeneration research

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Selective sparing of goblet cells and paneth cells in the intestine of methotrexate-treated rats. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: American journal of physiology. Gastrointestinal a

VOLUME: 279

Page Numbers: G1037-47

Journal Abbreviation: Am. J. Physiol. Gastrointest.

ISSN: 0193-1857

DAY: 30

MONTH: Nov

YEAR: 2000

Selective sparing of goblet cells and paneth cells in the intestine of methotrexate-treated rats. Information

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LANGUAGE: eng

NlmUniqueID: 100901227

Selective sparing of goblet cells and paneth cells in the intestine of methotrexate-treated rats. Keywords Mesh Terms:

KEYWORDS: Regeneration

MESH TERMS: physiology

Chemical & Substance for Abstract: Selective sparing of goblet cells and paneth cells in the intestine of methotrexate-treated rats. Information

Substance Name: Methotrexate

Registry Number: 59-05-2

Grant and Affiliation Information for Selective sparing of goblet cells and paneth cells in the intestine of methotrexate-treated rats.

AFFILIATION: Laboratory of Pediatric Gastroenterology and Nutrition, Erasmus University and Sophia Children's Hospital, 3015GE Rotterdam, The Netherlands.

Country: UNITED STATES

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MEDLINETA: Am J Physiol Gastrointest Live

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