Selective precipitation of prions by polyoxometalate complexes.

Selective precipitation of prions by polyoxometalate complexes. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Selective precipitation of prions by polyoxometalate complexes. Abstract Text:

In Su Lee,Jeffrey R Long,Stanley B Prusiner,Jiri G Safar,

The efficacy of a variety of polyoxometalate anions in selectively precipitating the infectious isoform of the prion protein (PrPSc) over the normal, cellular protein (PrPC) was tested as a means of improving the sensitivity of a conformation-dependent immunoassay (CDI). Experiments utilizing neutralized aqueous solutions containing MgCl2.6H2O and Na2H[PW12O40] (1) indicated that use of the former is detrimental to selectivity, while use of the latter gives optimal selectivity at a concentration of 2.48% w/v, much higher than the 0.31% w/v employed in the current CDI protocol. Similar experiments using Na4[SiW12O40] (2), K5[BW12O40] (3), and Na6[H2W12O40] (4) were performed to probe the role of charge density in polyoxometalate anions possessing the Keggin structure. These, coupled with assignment of solution structures via NMR and infrared spectroscopy, revealed the following trend in optimal concentrations: [HBW11O39]8- < [SiW11O39]8- < [PW11O39]7- = [H2W12O40]6-. Interestingly, use of the salts K10[Zn4(H2O)2(PW9O34)2] (5), Na16[Zn4(H2O)2(P2W15O56)2] (6), and Na27[NaAs4W40O140] (7), containing larger polyoxometalate anions, was found to suppress PrPSc precipitation in a concentration-dependent manner. Taken together, the results suggest a precipitation mechanism wherein Keggin-type anions link multiple PrPSc moieties by binding a somewhat hindered cleft featuring one or more positively charged residues.

Selective precipitation of prions by polyoxometalate complexes. Publishing Authors By Initials

IS Lee,JR Long,SB Prusiner,JG Safar,

For similar inorganic chemicals: elements: metals, heavy: zinc research abstracts see: inorganic chemicals: elements: metals, heavy: zinc research

PUBMED ID PMID:

MEDLINE DATE:

Selective precipitation of prions by polyoxometalate complexes. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of the American Chemical Society

VOLUME: 127

Page Numbers: 13802-3

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 12

MONTH: Oct

YEAR: 2005

Selective precipitation of prions by polyoxometalate complexes. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7503056

Selective precipitation of prions by polyoxometalate complexes. Keywords Mesh Terms:

KEYWORDS: Zinc

MESH TERMS: chemistry

Chemical & Substance for Abstract: Selective precipitation of prions by polyoxometalate complexes. Information

Substance Name: Zinc

Registry Number: 7440-66-6

Grant and Affiliation Information for Selective precipitation of prions by polyoxometalate complexes.

AFFILIATION: Department of Chemistry, University of California, Berkeley, California 94720-1460, USA.

Country: United States

AGENCY: United States NINDS

GRANT: NS02328

ACRONYM: NS

MEDLINETA: J Am Chem Soc

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Selective precipitation of prions by polyoxometalate complexes Related Publications

• A preliminary evaluation of the mean arterial pressure as measured by cuff oscillometry.
• A study of deliberate hypotension in anesthesia, with special consideration of surgical blood loss in comparable groups of normotensive and hypotensive anesthesia.
• Algotherapy in rehabilitation.
• An indirect evaluation of the effect of the autonomic nervous system following converting enzyme inhibition in hypertension.
• Angiotensin converting enzyme inhibition, pulse wave velocity and ambulatory blood pressure measurements in essential hypertension.
• Arterial and cardiac changes in hypertension in the elderly.
• Blood pressure and serum lipids in hypertensive men and women aged 60-97 years.
• Breeding for scrapie resistance in the Hungarian sheep population.
• Central hypotensive effects of nicardipine in conscious freely moving spontaneously hypertensive rat.
• Effects of chronic converting enzyme inhibition on the structure and function of large arteries in the rat.
• Homocysteine levels and peripheral arterial occlusive disease: a prospective cohort study and review of the literature.
• Impact of abdominal adiposity on cardiovascular disease predictors: what is the missing link?
• Not Available
• Placebo-controlled crossover comparison of spirapril at 3, 6, 12 and 24 mg once daily in mild to severe essential hypertension.
• Pulse pressure amplification, adiposity and metabolic syndrome in subjects under chronic antihypertensive therapy: The role of heart rate.
• Pulse pressure and dual Angiotensin blockade.
• Response to "spotlights on ambulatory measures of arterial stiffness".
• Selective precipitation of prions by polyoxometalate complexes.
• Sex-dependence of body fat distribution in patients with obesity and hypertension.
• The vibratory pattern of ultrasonic files driven piezoelectrically.
• Urinary Aldosterone-to-active-renin Ratio: A Useful Tool for Predicting Resolution of Hypertension After Adrenalectomy in Patients With Aldosterone-producing Adenomas.
• [Anesthesia for major abdominal surgery in patients in poor physical condition: combination of superficial general anesthesia with bilateral intercostal nerve block.]
• [Electrosurgery of malignant eye tumors.]
• [Not Available.]
• [Not Available.]
• [Not Available.]
• [Pulse pressure and cardiovascular risk]