Selective modulation of scleral proteoglycan mRNA levels during minus lens compensation and recovery.

Selective modulation of scleral proteoglycan mRNA levels during minus lens compensation and recovery. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Selective modulation of scleral proteoglycan mRNA levels during minus lens compensation and recovery. Abstract Text:

John Thomas Siegwart,Christianne E Strang,

PURPOSE: To better characterize the role of proteoglycans in scleral tissue remodeling during the development of minus lens induced myopia and during recovery in tree shrews. METHODS: Competitive reverse-transcription polymerase chain reaction (RT-PCR) was used to quantify the scleral mRNA levels for aggrecan, decorin, biglycan, and lumican in a group of tree shrews following four days of monocular -5 D lens treatment (n=5) and in a group after two days of recovery after 11 days of -5 D lens wear (n=5). Values were compared with age-matched normal animals (n=5). Aggrecan was localized within the sclera using immunohistochemistry. RESULTS: Four days of -5 D lens wear produced axial (vitreous chamber) elongation and a myopic shift in the treated eyes. Two days of recovery produced significant refractive recovery. Aggrecan mRNA levels showed differential, bidirectional regulation. Levels in the treated eye sclera relative to the control eye were 30.8%+/-2.4% lower after four days of -5 D lens treatment and 51.4%+/-2.4% higher after two days of recovery. Decorin, biglycan, and lumican mRNA levels showed little differential regulation. However, biglycan and lumican along with aggrecan showed binocular regulation (treated and control eye mRNA levels significantly lower than normal eye mRNA levels after 4 days of -5D lens treatment). Immunohistochemical results showed that aggrecan is present in tree shrew sclera and that it is located primarily between the collagen lamella and near the fibroblasts. CONCLUSIONS: These data suggest that the expression of aggrecan is strongly differentially modulated in the sclera during experimentally induced myopia and recovery. The modulation of aggrecan in concert with previously described changes in type I collagen and hyaluronan may play a key functional role in modulating the ability of the lamellae to slip across one another. This may be manifested in the scleral creep rate, which in turn modulates axial elongation rate and refractive state.

Selective modulation of scleral proteoglycan mRNA levels during minus lens compensation and recovery. Publishing Authors By Initials

JT Siegwart,CE Strang,

For similar animals: chordata: vertebrates: mammals: scandentia: tupaiidae research abstracts see: animals: chordata: vertebrates: mammals: scandentia: tupaiidae research

PUBMED ID PMID:

MEDLINE DATE:

Selective modulation of scleral proteoglycan mRNA levels during minus lens compensation and recovery. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Molecular vision

VOLUME: 13

Page Numbers: 1878-86

Journal Abbreviation: Mol. Vis.

ISSN: 1090-0535

DAY: 4

MONTH: 10

YEAR: 2007

Selective modulation of scleral proteoglycan mRNA levels during minus lens compensation and recovery. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9605351

Selective modulation of scleral proteoglycan mRNA levels during minus lens compensation and recovery. Keywords Mesh Terms:

KEYWORDS: Tupaiidae

MESH TERMS: metabolism

Chemical & Substance for Abstract: Selective modulation of scleral proteoglycan mRNA levels during minus lens compensation and recovery. Information

Substance Name: Keratan Sulfate

Registry Number: 9056-36-4

Grant and Affiliation Information for Selective modulation of scleral proteoglycan mRNA levels during minus lens compensation and recovery.

AFFILIATION: Department of Vision Sciences, School of Optometry, The University of Alabama at Birmingham, Birmingham, AL, USA. siegwart@uab.edu

Country: United States

AGENCY: United States NEI

GRANT: R01 EY07845

ACRONYM: EY

MEDLINETA: Mol Vis

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Selective modulation of scleral proteoglycan mRNA levels during minus lens compensation and recovery Related Publications

• 'Horse trading': prescribing injectable opiates to opiate addicts. A descriptive study.
• A "harlequin" colour change in the newborn.
• Community pharmacies and the provision of opioid substitution services for drug misusers: changes in activity and attitudes of community pharmacists across England 1995-2005.
• Confusion between the drug legalization and the drug prescribing debate.
• Estimation of K(m) values of enzymes requiring molecular O(2) as a substrate.
• GOLDEN ANNIVERSARY OF DENTAL HYGIENE.
• Gs18p case series: obturator hernia.
• High blood pressure in children is paralleled by an elevated heart rate during exercise: 1533: board #23 may 30 3:30 PM - 5:00 PM.
• Improved reliability of the Standardized Alzheimer's Disease Assessment Scale (SADAS) compared with the Alzheimer's Disease Assessment Scale (ADAS).
• Intraventricular hemorrhage caused by intraventricular meningioma: CT appearance.
• LYMPH-FLOW AND CLEARANCE OF LIQUID FROM THE LUNGS OF THE FOETAL LAMB.
• Measuring capacity to complete an advance directive.
• Multiple risks for HIV and hepatitis B infection among heroin users.
• PUBLIC HEALTH LABORATORY NOTES.
• PUBLIC HEALTH LABORATORY NOTES.
• PUBLIC HEALTH LABORATORY NOTES.
• PUBLIC HEALTH LABORATORY NOTES.
• PUBLIC HEALTH LABORATORY NOTES.
• PUBLIC HEALTH LABORATORY NOTES.
• PUBLIC HEALTH LABORATORY NOTES.
• PUBLIC HEALTH LABORATORY NOTES.
• PUBLIC HEALTH LABORATORY NOTES.
• PUBLIC HEALTH LABORATORY NOTES.
• PUBLIC HEALTH LABORATORY NOTES.
• PUBLIC HEALTH LABORATORY NOTES.
• Pharmacodynamics of diazepam co-administered with methadone or buprenorphine under high dose conditions in opioid dependent patients.
• Selective modulation of scleral proteoglycan mRNA levels during minus lens compensation and recovery.
• Techniques of Counseling in Regard to Health Problems.
• Techniques of counseling in regard to health problems.
• Thoracic intervertebral disk protrusions.