Selective labeling and isolation of functional classes of interstitial cells of Cajal of human and murine small intestine.

Selective labeling and isolation of functional classes of interstitial cells of Cajal of human and murine small intestine. Research Abstract Details 

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Selective labeling and isolation of functional classes of interstitial cells of Cajal of human and murine small intestine. Abstract Text:

Hui Chen,Doug Redelman,Seungil Ro,Sean M Ward, ,Kenton M Sanders,

Specific functions of interstitial cells of Cajal (ICC) have been linked to distinct classes that differ by morphology and distribution. In the small intestine, slow wave-generating ICC are located in the myenteric region (ICC-MY), whereas ICC that mediate neuromuscular neurotransmission occur either throughout the circular muscle layer (intramuscular ICC, ICC-IM) or in association with the deep muscular plexus (ICC-DMP). Selective isolation of ICC to characterize specific properties has been difficult. Recently, neurokinin-1 receptors have been detected in murine ICC-DMP and neurons but not in ICC-MY. Here we identified and isolated ICC-DMP/IM by receptor-mediated internalization of fluorescent substance P and Kit immunofluorescence. Specificity of labeling was verified by confocal microscopy. Mouse and human ICC-DMP/IM were detected in suspension by fluorescent microscopy and harvested for RT-PCR with micropipettes. The isolated cells expressed Kit but not markers for neurons, smooth muscle, or antigen-presenting cells. ICC-DMP expressed neurokinin-1 receptor, M(2) and M(3) muscarinic receptors, P2Y(1) and P2Y(4) purinergic receptors, VIP receptor 2, soluble guanylate cyclase-1 subunits, and protein kinase G. L- or T-type Ca(2+) channels were not detected in these cells. ICC-MY and ICC-DMP were simultaneously detected and enumerated by flow cytometry and sorted to purity by fluorescence-activated cell sorting. In summary, functional classes of ICC have distinct molecular identities that can be used to selectively identify and harvest these cells with, for example, receptor-mediated uptake of substance P and Kit immunofluorescence. ICC-DMP express neurotransmitter receptors and signaling intermediate molecules that are consistent with their role in neuromuscular neurotransmission.

Selective labeling and isolation of functional classes of interstitial cells of Cajal of human and murine small intestine. Publishing Authors By Initials

H Chen,D Redelman,S Ro,SM Ward,T ,KM Sanders,

For similar peptides: intercellular signaling peptides and proteins: kinins: tachykinins: substance p research abstracts see: peptides: intercellular signaling peptides and proteins: kinins: tachykinins: substance p research

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MEDLINE DATE:

Selective labeling and isolation of functional classes of interstitial cells of Cajal of human and murine small intestine. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: American journal of physiology. Cell physiology

VOLUME: 292

Page Numbers: C497-507

Journal Abbreviation: Am. J. Physiol., Cell Physiol.

ISSN: 0363-6143

DAY: 30

MONTH: 08

YEAR: 2006

Selective labeling and isolation of functional classes of interstitial cells of Cajal of human and murine small intestine. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100901225

Selective labeling and isolation of functional classes of interstitial cells of Cajal of human and murine small intestine. Keywords Mesh Terms:

KEYWORDS: Substance P

MESH TERMS: metabolism

Chemical & Substance for Abstract: Selective labeling and isolation of functional classes of interstitial cells of Cajal of human and murine small intestine. Information

Substance Name: Proto-Oncogene Proteins c-kit

Registry Number: EC 2.7.1.112

Grant and Affiliation Information for Selective labeling and isolation of functional classes of interstitial cells of Cajal of human and murine small intestine.

AFFILIATION: Department of Physiology and Cell Biology, School of Medicine, University of Nevada-Reno, Reno, NV 89557, USA.

Country: United States

AGENCY: United States NCRR

GRANT: P20 RR 16464

ACRONYM: RR

MEDLINETA: Am J Physiol Cell Physiol

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