Selective identification of HLA-DP4 binding T cell epitopes encoded by the MAGE-A gene family.

Selective identification of HLA-DP4 binding T cell epitopes encoded by the MAGE-A gene family. Research Abstract Details 

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Selective identification of HLA-DP4 binding T cell epitopes encoded by the MAGE-A gene family. Abstract Text:

Xiao-Fei Wang,William M Cohen,Florence A Castelli,Christine Almunia,Bernard ,Sandra Pouvelle-Moratille,Gaetan Munier,Dominique Charron, ,Hassan M Zarour,Pierre van der Bruggen,Marc Busson,Bernard ,

Because of the high frequency of HLA-DP4 in the Caucasian population, we have selectively delineated HLA-DP4 restricted T cell epitopes in the MAGE-A tumor antigens. We identified 12 good binders to HLA-DP4 and investigated the capacity of the seven best binders to induce in vitro specific CD4+ T cell lines from HLA-DP4 healthy donors. We found that the MAGE-A1 90-104 peptide exhibited a high and constant frequency of CD4+ T cell precursors in all the six tested donors. The MAGE-A1 268-282 peptide was found immunogenic in only two donors but with a high precursor frequency. The MAGE-A12 127-141 peptide was T cell stimulating in six different donors and induced fewer T cell lines. The peptide-specific T cell lines were stimulated by DC loaded with the lysates of cells transfected with MAGE-A1 or MAGE-A12, or loaded with the recombinant protein. We also show that the immunoreactivity of CD4+ T cell epitopes restricted to the same HLA II molecule may vary from one individual to another, as a result of inter-individual variations in the CD4+ T cell repertoire.

Selective identification of HLA-DP4 binding T cell epitopes encoded by the MAGE-A gene family. Publishing Authors By Initials

XF Wang,WM Cohen,FA Castelli,C Almunia,B ,S Pouvelle-Moratille,G Munier,D Charron,A ,HM Zarour,P van der Bruggen,M Busson,B ,

For similar proteins: neoplasm proteins research abstracts see: proteins: neoplasm proteins research

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Selective identification of HLA-DP4 binding T cell epitopes encoded by the MAGE-A gene family. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Cancer immunology, immunotherapy : CII

VOLUME: 56

Page Numbers: 807-18

Journal Abbreviation: Cancer Immunol. Immunother.

ISSN: 0340-7004

DAY: 20

MONTH: 09

YEAR: 2006

Selective identification of HLA-DP4 binding T cell epitopes encoded by the MAGE-A gene family. Information

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LANGUAGE: eng

NlmUniqueID: 8605732

Selective identification of HLA-DP4 binding T cell epitopes encoded by the MAGE-A gene family. Keywords Mesh Terms:

KEYWORDS: Neoplasm Proteins

MESH TERMS: immunology

Chemical & Substance for Abstract: Selective identification of HLA-DP4 binding T cell epitopes encoded by the MAGE-A gene family. Information

Substance Name: melanoma-specific antigens

Registry Number: 0

Grant and Affiliation Information for Selective identification of HLA-DP4 binding T cell epitopes encoded by the MAGE-A gene family.

AFFILIATION: Protein Engineering and Research Department, CEA-Saclay, 91191 Gif sur Yvette, France.

Country: Germany

AGENCY: United States NCI

GRANT: CA90360

ACRONYM: CA

MEDLINETA: Cancer Immunol Immunother

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